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Antimicrobial Agents and Chemotherapy, June 2002, p. 1977-1979, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1977-1979.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of the vanE Gene Cluster in Vancomycin-Resistant Enterococcus faecalis N00-410 Isolated in Canada

D. A. Boyd,1 T. Cabral,2 P. Van Caeseele,2 J. Wylie,2 and M. R. Mulvey1*

Nosocomial Infections, National Microbiology Laboratory, Health Canada,1 Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada2

Received 15 October 2001/ Returned for modification 14 January 2002/ Accepted 7 March 2002

The vanE operon was characterized from Enterococcus faecalis N00-410 (MIC of vancomycin = 24 µg/ml). The organization of the vanE operon was identical to that of the vanC1 operon from Enterococcus gallinarum, with protein identities ranging from 46 to 63%. An open reading frame located downstream of the vanE operon showed significant homology to a number of integrase genes, all of which are located downstream of the chromosomal GMP synthase gene guaA.

* Corresponding author. Mailing address: Nosocomial Infections, National Microbiology Laboratory, Health Canada, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-5020. E-mail: michael_mulvey{at}hc-sc.gc.ca.

Antimicrobial Agents and Chemotherapy, June 2002, p. 1977-1979, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1977-1979.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.