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Antimicrobial Agents and Chemotherapy, August 2002, p. 2337-2343, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2337-2343.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Tn5406, a New Staphylococcal Transposon Conferring Resistance to Streptogramin A and Related Compounds Including Dalfopristin

Julien Haroche, Jeanine Allignet, and Névine El Solh*

Staphylococci Unit, Institut Pasteur, 75724 Paris Cedex 15, France

Received 5 November 2001/ Returned for modification 11 February 2002/ Accepted 16 April 2002

We characterized a new transposon, Tn5406 (5,467 bp), in a clinical isolate of Staphylococcus aureus (BM3327). It carries a variant of vgaA, which encodes a putative ABC protein conferring resistance to streptogramin A but not to mixtures of streptogramins A and B. It also carries three putative genes, the products of which exhibit significant similarities (61 to 73% amino acid identity) to the three transposases of the staphylococcal transposon Tn554. Like Tn554, Tn5406 failed to generate target repeats. In BM3327, the single copy of Tn5406 was inserted into the chromosomal att554 site, which is the preferential insertion site of Tn554. In three other independent S. aureus clinical isolates, Tn5406 was either present as a single plasmid copy (BM3318), as two chromosomal copies (BM3252), or both in the chromosome and on a plasmid (BM3385). The Tn5406-carrying plasmids also contain two other genes, vgaB and vatB. The insertion sites of Tn5406 in BM3252 were studied: one copy was in att554, and one copy was in the additional SCCmec element. Amplification experiments revealed circular forms of Tn5406, indicating that this transposon might be active. To our knowledge, a transposon conferring resistance to streptogramin A and related compounds has not been previously described.

* Corresponding author. Mailing address: Staphylococci Unit, Institut Pasteur, 25/28 Rue du Docteur Roux 75724 Paris Cedex 15, France. Phone: (33) 01-45-68-83-63. Fax: (33) 01-40-61-31-63. Email: nelsolh{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2337-2343, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2337-2343.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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