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Antimicrobial Agents and Chemotherapy, August 2002, p. 2513-2517, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2513-2517.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children

Vicki A. Luna,1 Marc Heiken,1 Kathleen Judge,1 Catherine Ulep,1 Nicole Van Kirk,1 Henrique Luis,2 Mario Bernardo,2 Jose Leitao,2 and Marilyn C. Roberts1*

Department of Pathobiology, University of Washington, Seattle, Washington 98195,1 School of Dentistry, University of Lisbon, Lisbon, Portugal2

Received 7 December 2001/ Returned for modification 24 March 2002/ Accepted 4 May 2002

We screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(A), erm(B), erm(C), erm(F), and mef(A) genes. The mef(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including Enterococcus spp., Staphylococcus spp., and Streptococcus spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn1207.1, a genetic element carrying mef(A) recently described in Streptococcus pneumoniae. The three Staphylococcus spp. did not carry orf1 to orf3. A gram-negative Acinetobacter junii isolate also carried the other seven ORFs described in Tn1207.1. A Staphylococcus aureus isolate, a Streptococcus intermedius isolate, a Streptococcus sp. isolate, and an Enterococcus sp. isolate had their mef(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the mef(A) gene from S. pneumoniae.

* Corresponding author. Mailing address: Department of Pathobiology, Box 357238, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195. Phone: (206) 543-8001. Fax: (206) 543-3873. E-mail: marilynr{at}u.washington.edu.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2513-2517, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2513-2517.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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