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Antimicrobial Agents and Chemotherapy, August 2002, p. 2684-2686, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2684-2686.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Detection of a Molecular Marker for Chloroquine-Resistant Falciparum Malaria

Rémy Durand,* Virginie Huart, Sayeh Jafari, and Jacques Le Bras

Centre National de Référence pour la Chimiosensibilité du Paludisme, Assistance Publique-Hôpitaux de Paris, Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 75877 Paris Cedex 18, France

Received 12 March 2002/ Returned for modification 11 April 2002/ Accepted 15 May 2002

A PCR-based technique using molecular beacons was developed to detect the chloroquine resistance-associated pfcrt K76T point mutation in Plasmodium falciparum. One hundred thirty African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This rapid and inexpensive genomic assay could expand the possibilities for monitoring chloroquine resistance.

* Corresponding author. Mailing address: Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75877 Paris Cedex 18, France. Phone: 33 1 40 25 78 81. Fax: 33 1 40 25 67 63. E-mail: remy.durand{at}bch.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2684-2686, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2684-2686.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bertin, G., Ndam, N. T., Jafari-Guemouri, S., Fievet, N., Renart, E., Sow, S., Le Hesran, J.-Y., Deloron, P. (2005). High prevalence of Plasmodium falciparum pfcrt K76T mutation in pregnant women taking chloroquine prophylaxis in Senegal. J Antimicrob Chemother 55: 788-791 [Abstract] [Full Text]  


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