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Antimicrobial Agents and Chemotherapy, August 2002, p. 2712-2715, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2712-2715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Topoisomerase Mutations Associated with In Vitro Selection of Resistance to Moxifloxacin in Streptococcus pneumoniae

Serge Houssaye,1 Laurent Gutmann,1,2 and Emmanuelle Varon1,2*

L.R.M.A., INSERM E0004 Université Paris VI, 75270 Paris cedex 06,1 C.N.R. des Pneumocoques, Hôpital Européen Georges Pompidou, 75908 Paris cedex 15, France2

Received 15 January 2002/ Returned for modification 4 March 2002/ Accepted 9 May 2002

We analyzed the frequencies of selection, the order of acquisition, and the mutations selected on moxifloxacin in two wild-type pneumococcal strains, R6 and 5714. The first selection step showed either a single GyrA mutation or no mutation in any of the quinolone resistance-determining regions. Second-step mutants selected had either a second mutation in ParC or in ParE. Moxifloxacin could belong to these fluoroquinolones, which preferentially target GyrA though probably acting equally through both gyrase and topoisomerase IV.

* Corresponding author. Mailing address: Centre National de Référence des Pneumocoques, Hôpital Européen Georges Pompidou, 20-40 rue Leblanc, 75908 Paris cedex 15, France. Phone: 33-1-56 09 39 51. Fax: 33-1-56 09 24 46. E-mail: emmanuelle.varon{at}hop.egp.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2712-2715, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2712-2715.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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