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Antimicrobial Agents and Chemotherapy, August 2002, p. 2720-2722, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2720-2722.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino,1* Anandi Martin,1 Mirtha Camacho,2 Humberto Guerra,3 Jean Swings,4,5 and Françoise Portaels1

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000,1 Laboratorium voor Microbiologie,4 BCCM/LMG Culture Collection, Universiteit Gent, B-9000 Ghent, Belgium,5 Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, La Paz, Bolivia,2 Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima 100, Peru3

Received 8 February 2002/ Returned for modification 1 April 2002/ Accepted 1 May 2002

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, Antwerp 2000, Belgium. Phone: 32-3 2476334. Fax: 32-3 2476333. E-mail: palomino{at}itg.be.

Antimicrobial Agents and Chemotherapy, August 2002, p. 2720-2722, Vol. 46, No. 8
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.8.2720-2722.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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