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Antimicrobial Agents and Chemotherapy, September 2002, p. 2977-2981, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.2977-2981.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of a Self-Transferable Plasmid from Salmonella enterica Serotype Typhimurium Clinical Isolates Carrying Two Integron-Borne Gene Cassettes Together with Virulence and Drug Resistance Genes

Beatriz Guerra,1,2 Sara Soto,1 Reiner Helmuth,2 and M. Carmen Mendoza1*

Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, 33006 Oviedo, Principality of Asturias, Spain,1 Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), National Salmonella Reference Laboratory, 12254 Berlin, Germany2

Received 30 July 2001/ Returned for modification 29 November 2001/ Accepted 20 June 2002

An unusual self-transferable virulence-resistance plasmid (pUO-StVR2) was found in nine multidrug-resistant (ACSSuT phenotype) Salmonella enterica serotype Typhimurium clinical isolates that were assigned to four different phage types and a single and distinctive XbaI pulsed-field gel electrophoresis profile. pUO-StVR2 is an IncFII plasmid of about 140 kb in length carrying the spvA, spvB, and spvC (Salmonella plasmid virulence) and rck (resistance to complement killing) genes. It also carries the oxa1/aadA1a (ampicillin resistance and streptomycin-spectinomycin resistance) gene cassette configuration located within a class 1 integron with qacE{Delta}1/sul1 (ammonium antiseptics resistance and sulfadiazine resistance); the transposon genes merA, tnpA, and tnpR (mercury resistance, transposase, and resolvase of Tn21, respectively); and the catA1 (chloramphenicol resistance) and tet(B) (tetracycline resistance) genes. The insertion of resistance genes into a Salmonella virulence plasmid constitutes a new and interesting example of plasmid evolution and presents a serious public health problem.

* Corresponding author. Mailing address: Departamento de Biología Funcional, Área de Microbiología, Facultad de Medicina, Universidad de Oviedo, c/Julián Clavería 6, 33006 Oviedo, Spain. Phone: 34-985103560. Fax: 34-985103148. E-mail: cmendoza{at}correo.uniovi.es.

Antimicrobial Agents and Chemotherapy, September 2002, p. 2977-2981, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.2977-2981.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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