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Antimicrobial Agents and Chemotherapy, September 2002, p. 3101-3103, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3101-3103.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Development of a Yeast Assay for Rapid Screening of Inhibitors of Human-Derived Pneumocystis carinii Dihydrofolate Reductase

Liang Ma,,{dagger} Qiuyao Jia,,{dagger} and Joseph A. Kovacs*

Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892

Received 20 March 2002/ Returned for modification 14 May 2002/ Accepted 17 June 2002

Human-derived Pneumocystis carinii dihydrofolate reductase (DHFR) was expressed in a Saccharomyces cerevisiae strain whose growth depends on complementation by this enzyme. We utilized a quantitative assay to measure the sensitivity of this yeast strain to DHFR inhibitors. This assay should be useful for identifying new inhibitors of human-derived P. carinii DHFR.

* Corresponding author. Mailing address: Building 10, Room 7D43, National Institutes of Health, 10 Center Dr., MSC 1662, Bethesda, MD 20892-1662. Phone: (301) 496-9907. Fax: (301) 402-1213. E-mail: jkovacs{at}nih.gov.

{dagger} Present address: Department of Biology, Loyola University at Chicago, Chicago, IL 60626.

Antimicrobial Agents and Chemotherapy, September 2002, p. 3101-3103, Vol. 46, No. 9
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.9.3101-3103.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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