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Antimicrobial Agents and Chemotherapy, January 2003, p. 378-382, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.378-382.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Drug Targeting Mycobacterium tuberculosis Cell Wall Synthesis: Development of a Microtiter Plate-Based Screen for UDP-Galactopyranose Mutase and Identification of an Inhibitor from a Uridine-Based Library

Michael S. Scherman,1 Katharine A. Winans,2,1 Richard J. Stern,1,2 Victoria Jones,1 Carolyn R. Bertozzi,2 and Michael R. McNeil1*

Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523,1 Departments of Chemistry and Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 947202

Received 29 May 2002/ Returned for modification 19 August 2002/ Accepted 17 October 2002

A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

* Corresponding author. Mailing address: Department of Microbiology, Colorado State University, Fort Collins, CO 80523. Phone: (970) 491-1784. Fax: (970) 491-1815. E-mail: MMcneil{at}colostate.edu.

{dagger} Present address: Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305.

{ddagger} Present address: Heska Corporation, Fort Collins, CO 80525.

Antimicrobial Agents and Chemotherapy, January 2003, p. 378-382, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.378-382.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Beverley, S. M., Owens, K. L., Showalter, M., Griffith, C. L., Doering, T. L., Jones, V. C., McNeil, M. R. (2005). Eukaryotic UDP-Galactopyranose Mutase (GLF Gene) in Microbial and Metazoal Pathogens. Eukaryot Cell 4: 1147-1154 [Abstract] [Full Text]  


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