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Antimicrobial Agents and Chemotherapy, January 2003, p. 387-389, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.387-389.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Site-Specific Mutagenesis Analysis of PBP 1A from a Penicillin-Cephalosporin-Resistant Pneumococcal Isolate

Anthony M. Smith1* and Keith P. Klugman1,2

Respiratory and Meningeal Pathogens Research Unit, Department of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, Johannesburg, South Africa,1 Departments of International Health and Infectious Diseases, Emory University, Atlanta, Georgia2

Received 10 July 2002/ Returned for modification 30 August 2002/ Accepted 3 October 2002

Our mutagenesis study has investigated all amino acid mutations in the penicillin-binding domain of PBP 1A from Hungarian pneumococcal isolate 3191 to determine the importance of every mutation in the development of penicillin and cefotaxime resistance. Our data reveal that mutations at amino acid positions 574 to 577 and position 539 cause penicillin and cefotaxime resistance.

* Corresponding author. Mailing address: Respiratory and Meningeal Pathogens Research Unit, Department of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 27-011-4899335. Fax: 27-011-4899332. E-mail: anthonys{at}mail.saimr.wits.ac.za.

Antimicrobial Agents and Chemotherapy, January 2003, p. 387-389, Vol. 47, No. 1
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.1.387-389.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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