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Antimicrobial Agents and Chemotherapy, October 2003, p. 3345-3348, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3345-3348.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Ciprofloxacin Transport by Chemoattractant-Activated Polymorphonuclear Leukocytes: Regulation by Priming and Protein Kinase C

John D. Walters1,2* and Robin J. Nakkula1

Section of Periodontology, College of Dentistry,1 Department of Molecular and Cellular Biochemistry, College of Medicine and Public Health, The Ohio State University Health Sciences Center, Columbus, Ohio2

Received 14 January 2003/ Returned for modification 8 May 2003/ Accepted 3 July 2003

At infection sites, polymorphonuclear leukocyte (PMN) function is enhanced ("primed") by granulocyte-macrophage colony-stimulating factor (GM-CSF) or lipopolysaccharide (LPS) and activated by formyl peptides. In this study, GM-CSF or LPS alone had no significant effects on PMN ciprofloxacin transport. Through a mechanism involving protein kinase C, activation by formyl-Met-Leu-Phe (fMLP) significantly decreased the Km of ciprofloxacin transport and enhanced ciprofloxacin accumulation. This effect was dramatically enhanced when PMNs were primed with GM-CSF or LPS prior to activation by fMLP.

* Corresponding author. Mailing address: College of Dentistry, The Ohio State University, 305 West 12th Ave., P.O. Box 182357, Columbus, OH 43218-2357. Phone: (614) 292-1169. Fax: (614) 292-2438. E-mail: walters.2{at}osu.edu.

Antimicrobial Agents and Chemotherapy, October 2003, p. 3345-3348, Vol. 47, No. 10
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.10.3345-3348.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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