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Antimicrobial Agents and Chemotherapy, November 2003, p. 3616-3619, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3616-3619.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method

Anandi Martin,1* Mirtha Camacho,2 Françoise Portaels,1 and Juan Carlos Palomino1

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium,1 Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, Miraflores, La Paz, Bolivia2

Received 17 April 2003/ Returned for modification 18 June 2003/ Accepted 1 July 2003

The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp B-2000, Belgium. Phone: 32-3 2476334. Fax: 32-3 2476333. E-mail: amartin{at}itg.be.

Antimicrobial Agents and Chemotherapy, November 2003, p. 3616-3619, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3616-3619.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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