This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sinclair, A.
Right arrow Articles by Woodford, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sinclair, A.
Right arrow Articles by Woodford, N.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2003, p. 3620-3622, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3620-3622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci

Alistair Sinclair,1 Catherine Arnold,2 and Neil Woodford1*

Antibiotic Resistance Monitoring and Reference Laboratory,1 Genomics, Proteomics and Bioinformatics Unit, Specialist and Reference Microbiology Division, Health Protection Agency—Colindale, London NW9 5HT, United Kingdom2

Received 16 June 2003/ Returned for modification 31 July 2003/ Accepted 14 August 2003

Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

* Corresponding author. Mailing address: ARMRL, SRMD, Health Protection Agency—Colindale, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44-20-8200-4400, ext. 4255. Fax: 44-20-8358-3292. E-mail: neil.woodford{at}hpa.org.uk.

Antimicrobial Agents and Chemotherapy, November 2003, p. 3620-3622, Vol. 47, No. 11
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.11.3620-3622.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Feng, J., Lupien, A., Gingras, H., Wasserscheid, J., Dewar, K., Legare, D., Ouellette, M. (2009). Genome sequencing of linezolid-resistant Streptococcus pneumoniae mutants reveals novel mechanisms of resistance. Genome Res 19: 1214-1223 [Abstract] [Full Text]  
  • Livermore, D. M., Mushtaq, S., Warner, M., Woodford, N. (2009). Activity of oxazolidinone TR-700 against linezolid-susceptible and -resistant staphylococci and enterococci. J Antimicrob Chemother 63: 713-715 [Abstract] [Full Text]  
  • Jones, C. H., Ruzin, A., Tuckman, M., Visalli, M. A., Petersen, P. J., Bradford, P. A. (2009). Pyrosequencing Using the Single-Nucleotide Polymorphism Protocol for Rapid Determination of TEM- and SHV-Type Extended-Spectrum {beta}-Lactamases in Clinical Isolates and Identification of the Novel {beta}-Lactamase Genes blaSHV-48, blaSHV-105, and blaTEM-155. Antimicrob. Agents Chemother. 53: 977-986 [Abstract] [Full Text]  
  • Shaw, K. J., Poppe, S., Schaadt, R., Brown-Driver, V., Finn, J., Pillar, C. M., Shinabarger, D., Zurenko, G. (2008). In Vitro Activity of TR-700, the Antibacterial Moiety of the Prodrug TR-701, against Linezolid-Resistant Strains. Antimicrob. Agents Chemother. 52: 4442-4447 [Abstract] [Full Text]  
  • Haanpera, M., Forssten, S. D., Huovinen, P., Jalava, J. (2008). Typing of SHV Extended-Spectrum {beta}-Lactamases by Pyrosequencing in Klebsiella pneumoniae Strains with Chromosomal SHV {beta}-Lactamase. Antimicrob. Agents Chemother. 52: 2632-2635 [Abstract] [Full Text]  
  • Moder, K.-A., Layer, F., Konig, W., Konig, B. (2007). Rapid screening of clarithromycin resistance in Helicobacter pylori by pyrosequencing. J Med Microbiol 56: 1370-1376 [Abstract] [Full Text]  
  • Haanpera, M., Jalava, J., Huovinen, P., Meurman, O., Rantakokko-Jalava, K. (2007). Identification of Alpha-Hemolytic Streptococci by Pyrosequencing the 16S rRNA Gene and by Use of VITEK 2. J. Clin. Microbiol. 45: 762-770 [Abstract] [Full Text]  
  • Livermore, D. M., Warner, M., Mushtaq, S., North, S., Woodford, N. (2007). In Vitro Activity of the Oxazolidinone RWJ-416457 against Linezolid-Resistant and -Susceptible Staphylococci and Enterococci. Antimicrob. Agents Chemother. 51: 1112-1114 [Abstract] [Full Text]  
  • Naas, T., Oxacelay, C., Nordmann, P. (2007). Identification of CTX-M-Type Extended-Spectrum-{beta}-Lactamase Genes Using Real-Time PCR and Pyrosequencing. Antimicrob. Agents Chemother. 51: 223-230 [Abstract] [Full Text]  
  • Poirel, L., Naas, T., Nordmann, P. (2006). Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum {beta}-Lactamases.. J. Clin. Microbiol. 44: 3008-3011 [Abstract] [Full Text]  
  • Jureen, P., Engstrand, L., Eriksson, S., Alderborn, A., Krabbe, M., Hoffner, S. E. (2006). Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis by Pyrosequencing Technology.. J. Clin. Microbiol. 44: 1925-1929 [Abstract] [Full Text]  
  • Bonora, M. G., Solbiati, M., Stepan, E., Zorzi, A., Luzzani, A., Catania, M. R., Fontana, R. (2006). Emergence of Linezolid Resistance in the Vancomycin-Resistant Enterococcus faecium Multilocus Sequence Typing C1 Epidemic Lineage.. J. Clin. Microbiol. 44: 1153-1155 [Abstract] [Full Text]  
  • Woodford, N., Sundsfjord, A. (2005). Molecular detection of antibiotic resistance: when and where?. J Antimicrob Chemother 56: 259-261 [Abstract] [Full Text]  
  • Werner, G., Strommenger, B., Klare, I., Witte, W. (2004). Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5' Nuclease Real-Time PCR Compared to a Modified Classical Approach. J. Clin. Microbiol. 42: 5327-5331 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»