This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Larabi, M.
Right arrow Articles by Barratt, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Larabi, M.
Right arrow Articles by Barratt, G.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 2003, p. 3774-3779, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3774-3779.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Toxicity and Antileishmanial Activity of a New Stable Lipid Suspension of Amphotericin B

Malika Larabi,1 Vanessa Yardley,2 Philippe M. Loiseau,3 Martine Appel,1 Philippe Legrand,1 Annette Gulik,4 Christian Bories,3 Simon L. Croft,2 and Gillian Barratt1*

Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612,1 Chimiothérapie Antiparasitaire, UMR 8076 CNRS, Faculté de Pharmacie, Université Paris XI, 92296 ChÂtenay Malabry Cedex,3 Centre de Génétique Moléculaire, UPR CNRS 9061, 91198 Gif-sur-Yvette Cedex, France,4 Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London WC1E 7HT, United Kingdom2

Received 7 May 2003/ Returned for modification 20 June 2003/ Accepted 30 August 2003

The aim of the present study was to evaluate the toxicity and the activity of a new lipid complex formulation of amphotericin B (AMB) (LC-AMB; dimyristoyl phosphatidylcholine, dimyristoyl phosphatidylglycerol, and AMB) that can be produced by a simple process. Like other lipid formulations, this new complex reduced both the hemolytic activity of AMB (the concentration causing 50% hemolysis of human erythrocytes, >100 µg/ml) and its toxicity toward murine peritoneal macrophages (50% inhibitory concentration, >100 µg/ml at 24 h). The in vivo toxicity of the new formulation (50% lethal dose, >200 mg/kg of body weight for CD1 mice) was similar to those of other commercial lipid formulations of AMB. The complex was the most effective formulation against the DD8 strain of Leishmania donovani. It was unable to reverse the resistance of an AMB-resistant L. donovani strain. In vivo LC-AMB was less efficient than AmBisome against L. donovani.

* Corresponding author. Mailing address: Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, UMR CNRS 8612, Faculty of Pharmacy, Université Paris XI, 5 rue Jean Baptiste Clément, 92296 ChÂtenay-Malabry Cedex, France. Phone: (33) (0) 1 46 83 56 27. Fax: (33) (0) 1 46 61 93 34. E-mail: Gillian.Barratt{at}cep.u-psud.fr.

Antimicrobial Agents and Chemotherapy, December 2003, p. 3774-3779, Vol. 47, No. 12
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.12.3774-3779.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Manandhar, K. D., Yadav, T. P., Prajapati, V. K., Kumar, S., Rai, M., Dube, A., Srivastava, O. N., Sundar, S. (2008). Antileishmanial activity of nano-amphotericin B deoxycholate. J Antimicrob Chemother 62: 376-380 [Abstract] [Full Text]  
  • Ponte-Sucre, A., Faber, J. H., Gulder, T., Kajahn, I., Pedersen, S. E. H., Schultheis, M., Bringmann, G., Moll, H. (2007). Activities of Naphthylisoquinoline Alkaloids and Synthetic Analogs against Leishmania major. Antimicrob. Agents Chemother. 51: 188-194 [Abstract] [Full Text]  
  • Charvalos, E., Tzatzarakis, M. N., Van Bambeke, F., Tulkens, P. M., Tsatsakis, A. M., Tzanakakis, G. N., Mingeot-Leclercq, M.-P. (2006). Water-soluble amphotericin B-polyvinylpyrrolidone complexes with maintained antifungal activity against Candida spp. and Aspergillus spp. and reduced haemolytic and cytotoxic effects. J Antimicrob Chemother 57: 236-244 [Abstract] [Full Text]  
  • Al-Mohammed, H. I., Chance, M. L., Bates, P. A. (2005). Production and Characterization of Stable Amphotericin-Resistant Amastigotes and Promastigotes of Leishmania mexicana. Antimicrob. Agents Chemother. 49: 3274-3280 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»