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Antimicrobial Agents and Chemotherapy, April 2003, p. 1423-1426, Vol. 47, No. 4
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.4.1423-1426.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Detection of Aminoglycoside Resistance Genes in Enterococci

Sergei B. Vakulenko,1,2 Susan M. Donabedian,3 Anatoliy M. Voskresenskiy,1 Marcus J. Zervos,3 Stephen A. Lerner,1,2 and Joseph W. Chow1,3,4*

Division of Infectious Diseases,1 Department of Biochemistry and Molecular Biology, Wayne State University,2 Research and Medical Services, John D. Dingell VA Medical Center, Detroit, Michigan 48201,4 Research Institute, William Beaumont Hospital, Royal Oak, Michigan 480733

Received 18 September 2002/ Returned for modification 20 December 2002/ Accepted 16 January 2003

A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.

* Corresponding author. Mailing address: Medical Service, John D. Dingell VA Medical Center, 4646 John R, Detroit, MI 48201-1932. Phone: (313) 576-3310. Fax: (313) 576-1122. E-mail: JChow{at}wayne.edu.

Antimicrobial Agents and Chemotherapy, April 2003, p. 1423-1426, Vol. 47, No. 4
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.4.1423-1426.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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