This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aouida, M.
Right arrow Articles by Mir, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aouida, M.
Right arrow Articles by Mir, L. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2003, p. 2012-2014, Vol. 47, No. 6
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.6.2012-2014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparative Roles of the Cell Wall and Cell Membrane in Limiting Uptake of Xenobiotic Molecules by Saccharomyces cerevisiae

Mustapha Aouida,1,2 Omar Tounekti,2,{dagger} Omrane Belhadj,2 and Lluis M. Mir1*

Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave-Roussy, 94805 Villejuif Cédex, France,1 Laboratoire de Biochimie et Technobiologie, Faculté des Sciences de Tunis, Campus Universitaire, 1060 Tunis, Tunisia2

Received 2 December 2002/ Accepted 5 March 2003

Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.

* Corresponding author. Mailing address: Laboratoire Vectorologie et Transfert de Gènes, UMR 8121 CNRS, Institut Gustave-Roussy, 39 rue Camille Desmoulins, 94805 Villejuif Cédex, France. Phone: 33 1 42 11 47 92. Fax: 33 1 42 11 52 45. E-mail: luismir{at}igr.fr.

{dagger} Present address: The Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital Montreal, QC H3T 1E2, Canada.

Antimicrobial Agents and Chemotherapy, June 2003, p. 2012-2014, Vol. 47, No. 6
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.6.2012-2014.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, M., Liang, Y., Zhang, X., Xu, Y., Dai, H., Xiao, W. (2008). Deletion of Yeast CWP Genes Enhances Cell Permeability to Genotoxic Agents. Toxicol Sci 103: 68-76 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»