This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubois, V.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubois, V.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2003, p. 2380-2381, Vol. 47, No. 7
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.7.2380-2381.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Decreased Susceptibility to Cefepime in a Clinical Strain of Escherichia coli Related to Plasmid- and Integron-Encoded OXA-30 ß-Lactamase


arrow
LETTER
 
The most frequent extended-spectrum ß-lactamases (ESBLs) in members of the family Enterobacteriaceae are derived from the clavulanic acid-inhibited Ambler class A enzymes TEM-1/TEM-2 and SHV-1, which may hydrolyze ceftazidime and cefepime (4, 5). The Ambler class B enzymes have the broadest hydrolysis profiles, including cefepime and ceftazidime (8), whereas several OXA-derived ESBLs (Ambler class D) usually hydrolyze more ceftazidime than cefepime (3). However, cefepime resistance combined with ceftazidime susceptibility has been detailed from Pseudomonas aeruginosa strains producing OXA-1-type enzyme (1). In this study, we describe a clinical isolate of Escherichia coli that was more resistant to cefepime than to ceftazidime due to another OXA-1 derivative, OXA-30.

Two strains of E. coli, Ec1484R and Ec1499S, were isolated from the urinary tract of a 70-year-old patient. This man was admitted to a neurological ward for a post-herpes zoster encephalopathy. He did not have any history of antimicrobial therapy. Randomly amplified polymorphic DNA (RAPD) with primer AP12h and enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR with primers ERIC2 and ERIC1R (10) gave the same pattern for Ec1484R and Ec1499S, compared to distinct and different profiles obtained with two unrelated E. coli strains used as controls (data not shown). According to MIC results, Ec1484R exhibited resistance to aminopenicillins and carboxypenicillins, alone or combined with ß-lactamase inhibitors, and showed a decreased susceptibility to cefepime, cefpirome, and cefotaxime, in contrast to susceptibility to ceftriaxone, cefoxitin, and ceftazidime (Table 1). This strain was also resistant to sulfonamides, streptomycin, and spectinomycin, which were cotransferred with ß-lactam resistance by the filter-mating technique to a rifampin- and nalidixic acid-resistant E. coli K12 strain (conjugation rate, 4 x 10-5). By isoelectric focusing analysis, Ec1484R and the transconjugant Tc1484 produced a single ß-lactamase with pI value of 7.3, and plasmid DNA analysis identified a large plasmid (size of >70 kb). Amplification with primers specific for blaOXA-1 (6) and subsequent sequencing revealed the presence of the blaOXA-30 gene, which was recently described for Shigella flexneri from Chinese isolates (11). Since most of the blaOXA genes are integron located, specific amplifications with integron-specific primers (9) and sequencing demonstrated that blaOXA-30 was part of the first cassette of a class 1 integron, adjacent to a second cassette containing the adenyltransferase-encoding gene aadA1 responsible for streptomycin and spectinomycin resistance (GenBank accession no. AY224185). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis did not show any evidence of outer membrane protein deficiency (data not shown). The blaOXA-30 gene was cloned into the pBK-CMV vector, and the resulting recombinant plasmid, pCOI, was expressed in E. coli TOP10. After ß-lactamase purification, performed as described previously (7), the specific activities of several ß-lactams were determined, indicating that OXA-30 hydrolyzed cefepime and cefpirome more than ceftazidime and cefotaxime (Table 2).


View this table:
[in this window]
[in a new window]
  		TABLE 1. MICs of ß-lactams for the E. coli strains used in this study


View this table:
[in this window]
[in a new window]
  		TABLE 2. Specific activities of OXA-30 expressed from the recombinant plasmid pCOI in E. coli TOP10

The present work is the first report of an E. coli clinical isolate in France that produces an OXA-1-like enzyme. Thus, this OXA-type enzyme may have also spread in members of the family Enterobacteriaceae, not only in P. aeruginosa (1, 2). Extensive use of cefepime may lead to the emergence of such an enzyme and subsequently select cefepim-resistant Enterobacteriaceae.


arrow
REFERENCES
 
    1
  1. Aubert, D., L. Poirel, J. Chevalier, S. Leotard, J.-M. Pages, and P. Nordmann. 2001. Oxacillinase-mediated resistance to cefepime and susceptibility to ceftazidime in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45:1615-1620.[Abstract/Free Full Text]
    2. 2
  2. Marumo, K., A. Takeda, Y. Nakamura, and K. Nakaya. 1999. Detection of OXA-4 ß-lactamase in Pseudomonas aeruginosa isolates by genetic methods. J. Antimicrob. Chemother. 43:187-193.[Abstract/Free Full Text]
    3. 3
  3. Naas, T., and P. Nordmann. 1999. OXA-type ß-lactamases. Curr. Pharm. Des. 5:865-879.[Medline]
    4. 4
  4. Nordmann, P. 1998. Trends in ß-lactam resistance among Enterobacteriaceae. Clin. Infect. Dis. 27:S100-S106.
    5. 5
  5. Nordmann, P., and M. Guibert. 1998. Extended-spectrum ß-lactamases in Pseudomonas aeruginosa. J. Antimicrob. Chemother. 42:128-131.[Free Full Text]
    6. 6
  6. Ouellette, M., L. Bissonnette, and P. H. Roy. 1987. Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 ß-lactamase gene. Proc. Natl. Acad. Sci. USA 84:7378-7382.[Abstract/Free Full Text]
    7. 7
  7. Poirel, L., P. Gerome, C. De Champs, J. Stephanazzi, T. Naas, and P. Nordmann. 2002. Integron-located oxa-32 gene cassette encoding an extended-spectrum variant of OXA-2 ß-lactamase from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46:566-569.[Abstract/Free Full Text]
    8. 8
  8. Poirel, L., and P. Nordmann. 2002. Acquired carbapenem-hydrolyzing ß-lactamases and their genetic support. Curr. Pharm. Biotechnol. 3:117-127.[CrossRef][Medline]
    9. 9
  9. Recchia, G. D., and R. M. Hall. 1995. Gene cassettes: a new class of mobile element. Microbiology 141:3015-3027.[Free Full Text]
    10. 10
  10. Renders, N., Y. Römling, H. Verbrugh, and A. van Belkum. 1996. Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. J. Clin. Microbiol. 34:3190-3195.[Abstract]
    11. 11
  11. Siu, L. K., J. Y. C. Lo, K. Y. Yuen, P. Y. Chau, M. H. Ng, and P. L. Ho. 2000. ß-Lactamases in Shigella flexneri isolates from Hong Kong and Shanghai and a novel OXA-1-like ß-lactamase, OXA-30. Antimicrob. Agents Chemother. 44:2034-2038.[Abstract/Free Full Text]
Véronique Dubois*
Corinne Arpin
Claudine Quentin
Laboratoire de Microbiologie
Faculté de Pharmacie
Université de Bordeaux 2
Bordeaux, France

Jeannette Texier-Maugein
Laboratoire de Bactériologie
Hôpital du Haut-Lévêque
Pessac France

Laurent Poirel
Patrice Nordmann
Service de Bactériologie-Virologie
Hôpital de Bicêtre
Assistance Publique/Hôpitaux de Paris
Faculté de Médecine Paris-Sud
Le Kremlin-Bicêtre, France

* Phone: 33 5 57 57 10 75
Fax: 33 5 56 90 90 72
E-mail: veronique.dubois{at}bacterio.u-bordeaux2.fr

Antimicrobial Agents and Chemotherapy, July 2003, p. 2380-2381, Vol. 47, No. 7
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.7.2380-2381.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hanson, N. D., Moland, E. S., Hong, S. G., Propst, K., Novak, D. J., Cavalieri, S. J. (2008). Surveillance of Community-Based Reservoirs Reveals the Presence of CTX-M, Imported AmpC, and OXA-30 {beta}-Lactamases in Urine Isolates of Klebsiella pneumoniae and Escherichia coli in a U.S. Community. Antimicrob. Agents Chemother. 52: 3814-3816 [Abstract] [Full Text]  
  • Bethel, C. R., Distler, A. M., Ruszczycky, M. W., Carey, M. P., Carey, P. R., Hujer, A. M., Taracila, M., Helfand, M. S., Thomson, J. M., Kalp, M., Anderson, V. E., Leonard, D. A., Hujer, K. M., Abe, T., Venkatesan, A. M., Mansour, T. S., Bonomo, R. A. (2008). Inhibition of OXA-1 {beta}-Lactamase by Penems. Antimicrob. Agents Chemother. 52: 3135-3143 [Abstract] [Full Text]  
  • Lascols, C., Podglajen, I., Verdet, C., Gautier, V., Gutmann, L., Soussy, C.-J., Collatz, E., Cambau, E. (2008). A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae. J. Bacteriol. 190: 5217-5223 [Abstract] [Full Text]  
  • Dubois, V., Parizano, M.-P., Arpin, C., Coulange, L., Bezian, M.-C., Quentin, C. (2007). High Genetic Stability of Integrons in Clinical Isolates of Shigella spp. of Worldwide Origin. Antimicrob. Agents Chemother. 51: 1333-1340 [Abstract] [Full Text]  
  • Pan, J.-C., Ye, R., Meng, D.-M., Zhang, W., Wang, H.-Q., Liu, K.-Z. (2006). Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri. J Antimicrob Chemother 58: 288-296 [Abstract] [Full Text]  
  • Chmelnitsky, I., Carmeli, Y., Leavitt, A., Schwaber, M. J., Navon-Venezia, S. (2005). CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple Escherichia coli Clones Isolated in Tel Aviv, Israel. Antimicrob. Agents Chemother. 49: 4745-4750 [Abstract] [Full Text]  
  • Moubareck, C., Doucet-Populaire, F., Hamze, M., Daoud, Z., Weill, F.-X. (2005). First Extended-Spectrum-{beta}-Lactamase (CTX-M-15)-Producing Salmonella enterica Serotype Typhimurium Isolate Identified in Lebanon. Antimicrob. Agents Chemother. 49: 864-865 [Full Text]  
  • Kim, Y.-R., Kim, S.-I., Lee, J.-Y., Park, Y.-J., Lee, K.-Y., Kang, M.-W. (2005). NosocomialTransmission of CTX-M-15 and OXA-30 {beta}-Lactamase-Producing Escherichia coli in a Neurosurgical Intensive Care Unit. Annals of Clinical & Laboratory Science 35: 297-301 [Abstract] [Full Text]  
  • Mammeri, H., Nazic, H., Naas, T., Poirel, L., Leotard, S., Nordmann, P. (2004). AmpC {beta}-Lactamase in an Escherichia coli Clinical Isolate Confers Resistance to Expanded-Spectrum Cephalosporins. Antimicrob. Agents Chemother. 48: 4050-4053 [Abstract] [Full Text]  
  • Antunes, P., Machado, J., Sousa, J. C., Peixe, L. (2004). Dissemination amongst humans and food products of animal origin of a Salmonella typhimurium clone expressing an integron-borne OXA-30 {beta}-lactamase. J Antimicrob Chemother 54: 429-434 [Abstract] [Full Text]  

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubois, V.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubois, V.
Right arrow Articles by Nordmann, P.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»