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Antimicrobial Agents and Chemotherapy, August 2003, p. 2572-2578, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2572-2578.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Extended-Spectrum ß-Lactamases in Ireland, Including a Novel Enzyme, TEM-102

Dearbháile Morris,1* Colette O'Hare,1 Maura Glennon,2 Majella Maher,2 Geraldine Corbett-Feeney,1 and Martin Cormican1

Department of Bacteriology,1 National Diagnostics Centre, National University of Ireland, Galway, Ireland2

Received 20 December 2002/ Returned for modification 3 March 2003/ Accepted 21 May 2003

Organisms producing extended-spectrum ß-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Ireland. A total of 925 isolates of ampicillin-resistant members of the Enterobacteriaceae were received from six hospitals in Ireland over a 3-year period from September 1996 to September 1999. Isolates were screened for ESBL production by the double-disk diffusion (DDD) method. DDD-positive isolates that were (i) confirmed as ESBL producers by National Committee for Clinical Laboratory Standards (NCCLS) confirmatory testing and (ii) susceptible to cefoxitin by disk diffusion were considered ESBL producers. By these criteria, 27 (3%) of the ampicillin-resistant members of the Enterobacteriaceae studied were categorized as ESBL producers. Molecular typing suggested that some intra- and interhospital spread of ESBL-producing isolates had occurred. DNA sequencing of amplified blaTEM and blaSHV genes resulted in the detection of a novel blaTEM ESBL gene, blaTEM-102 in two isolates (Klebsiella pneumoniae and Enterobacter cloacae) received from the same hospital but isolated from different patients. The study suggests dissemination of ESBL-producing bacteria within the health care system in Ireland and emphasizes the need for measures to control such spread.

* Corresponding author. Mailing address: Department of Bacteriology, National University of Ireland, Galway, Ireland. Phone: 353 91 544628. Fax: 353 91 512514. E-mail: dearbhailemorris{at}hotmail.com.

Antimicrobial Agents and Chemotherapy, August 2003, p. 2572-2578, Vol. 47, No. 8
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.8.2572-2578.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mugnaioli, C., Luzzaro, F., De Luca, F., Brigante, G., Perilli, M., Amicosante, G., Stefani, S., Toniolo, A., Rossolini, G. M. (2006). CTX-M-Type Extended-Spectrum {beta}-Lactamases in Italy: Molecular Epidemiology of an Emerging Countrywide Problem.. Antimicrob. Agents Chemother. 50: 2700-2706 [Abstract] [Full Text]  
  • Morris, D., Whelan, M., Corbett-Feeney, G., Cormican, M., Hawkey, P., Li, X., Doran, G. (2006). First Report of Extended-Spectrum-{beta}-Lactamase-Producing Salmonella enterica Isolates in Ireland.. Antimicrob. Agents Chemother. 50: 1608-1609 [Full Text]  


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