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Antimicrobial Agents and Chemotherapy, September 2003, p. 2938-2945, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2938-2945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Insertion Sequence ISEcp1B Is Involved in Expression and Mobilization of a blaCTX-M ß-Lactamase Gene

Laurent Poirel, Jean-Winoc Decousser, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin Bicêtre cedex, France

Received 14 April 2003/ Accepted 17 May 2003

The genetic structures (ca. 10-kb DNA fragment) surrounding the plasmid-borne extended-spectrum ß-lactamase blaCTX-M-19 gene in a Klebsiella pneumoniae clinical isolate were determined. This ß-lactamase gene was part of a 4,797-bp transposon inserted inside orf1 of Tn1721. Inside this transposon, blaCTX-M-19 was bracketed upstream and downstream by insertion sequences ISE cp1B and IS903D, respectively, and further downstream by a truncated gene encoding an outer membrane protein for iron transport. The single-copy ISEcp1B element was probably involved alone in the mobilization process that led to a 5-bp duplication at the target site of the transposed fragment. This mobilization event probably involved one inverted repeat of ISE cp1B and a second sequence farther away, resembling its second inverted repeat. Additionally, ISEcp1B provided -35 and -10 promoter sequences, contributing to the high-level expression of the blaCTX-M-19 gene. Southern blot analysis failed to identify a reservoir of ISEcp1-like sequences among a series of gram-negative and gram-positive bacterial species usually found in the skin and intestinal human floras. The ability of ISEcp1-like elements to mobilize and to promote the expression of ß-lactamase genes may explain, in part, the current spread of CTX-M-type enzymes worldwide.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2938-2945, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2938-2945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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