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Antimicrobial Agents and Chemotherapy, September 2003, p. 2962-2965, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2962-2965.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Gentamicin Delivery to Burkholderia cepacia Group IIIa Strains via Membrane Vesicles from Pseudomonas aeruginosa PAO1

Nick D. Allan and Terry J. Beveridge*

Canadian Bacterial Disease Network, National Centre of Excellence, Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Received 15 April 2003/ Returned for modification 5 May 2003/ Accepted 30 May 2003

When Pseudomonas aeruginosa PAO1 is treated with gentamicin, it releases membrane vesicles containing gentamicin (g-MVs) and peptidoglycan hydrolase, which makes the MVs bactericidal. We evaluate the ability of g-MVs to deliver gentamicin past the intrinsic permeability barrier of group IIIa Burkholderia cepacia and show that strain CEP0248 with low resistance to gentamicin is killed but the highly resistant strain C5424 is not. Immunoelectron microscopy revealed that gentamicin was delivered into both strains, suggesting that there might be another mechanism of resistance in C5424.

* Corresponding author. Mailing address: Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontairo, Canada N1G 2W1. Phone: (519) 824-4120, ext. 53366. Fax: (519) 837-1802. E-mail: tjb{at}uoguelph.ca.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2962-2965, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2962-2965.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kuehn, M. J., Kesty, N. C. (2005). Bacterial outer membrane vesicles and the host-pathogen interaction. Genes Dev. 19: 2645-2655 [Abstract] [Full Text]  


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