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Antimicrobial Agents and Chemotherapy, September 2003, p. 2990-2992, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2990-2992.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Functional Cloning and Characterization of a Multidrug Efflux Pump, MexHI-OpmD, from a Pseudomonas aeruginosa Mutant

Hiroshi Sekiya,1 Takehiko Mima,1 Yuji Morita,1 Teruo Kuroda,2 Tohru Mizushima,1 and Tomofusa Tsuchiya1*

Department of Microbiology, Faculty of Pharmaceutical Sciences,1 Gene Research Center, Okayama University, Tsushima, Okayama, 700-8530, Japan2

Received 25 February 2003/ Returned for modification 2 April 2003/ Accepted 14 June 2003

We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan. Phone and fax: 81-86-251-7957. E-mail: tsuchiya{at}pharm.okayama-u.ac.jp.

Antimicrobial Agents and Chemotherapy, September 2003, p. 2990-2992, Vol. 47, No. 9
0066-4804/03/$08.00+0     DOI: 10.1128/AAC.47.9.2990-2992.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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