This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boyd, D. A.
Right arrow Articles by Mulvey, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boyd, D. A.
Right arrow Articles by Mulvey, M. R.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, October 2004, p. 3758-3764, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.3758-3764.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Complete Nucleotide Sequence of a 92-Kilobase Plasmid Harboring the CTX-M-15 Extended-Spectrum Beta-Lactamase Involved in an Outbreak in Long-Term-Care Facilities in Toronto, Canada

David A. Boyd,1 Shaun Tyler,1 Sara Christianson,1 Allison McGeer,2 Matthew P. Muller,2 Barbara M. Willey,2 Elizabeth Bryce,3 Michael Gardam,4 Patrice Nordmann,5 Michael R. Mulvey,1* and Canadian Nosocomial Infection Surveillance Program, Health Canada{dagger}

Nosocomial Infections, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba,1 Mount Sinai Hospital,2 University Health Network, Toronto, Ontario,4 The Vancouver General Hospital, Vancouver, British Columbia, Canada,3 Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Paris, France5

Received 6 January 2004/ Returned for modification 21 April 2004/ Accepted 26 June 2004

A major outbreak involving an Escherichia coli strain that was resistant to expanded-spectrum cephalosporins occurred in Toronto and surrounding regions in 2000 to 2002. We report the complete sequence of a plasmid, pC15-1a, that was found associated with the outbreak strain. Plasmid pC15-1a is a circular molecule of 92,353 bp consisting of two distinct regions. The first is a 64-kb region that is essentially homologous to the non-R-determinant region of plasmid R100 except for several point mutations, a few small insertions and deletions, and the absence of Tn10. The second is a 28.4-kb multidrug resistance region (MDR) that has replaced the R-determinant region of the R100 progenitor and consists mostly of transposons or partial transposons and five copies of the insertion element IS26. All drug resistance genes found in pC15-1a, including the beta-lactamase genes blaCTX-M-15, blaOXA-1, and blaTEM-1, the tetracycline resistance gene tetA, and aminoglycoside resistance genes aac(6')-Ib and aac(3)-II, are located in the MDR. The blaCTX-M-15 gene was found downstream of ISEcp1as part of a transposition unit, as determined from the surrounding sequence. Examination of the plasmids from CTX-M-15-harboring strains isolated from hospitals across Canada showed that pC15-1a was found in several strains isolated from a site in western Canada. Comparison of pC15-1a and pCTX15, found in an E. coli strain isolated in India in 1999, revealed that the plasmids had several features in common, including an R100 backbone and several of the resistance genes, including blaCTX-M-15, blaTEM-1, blaOXA-1, tetA, and aac(6')-Ib.

* Corresponding author. Mailing address: Nosocomial Infections, National Microbiology Laboratory, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-2018. E-mail: michael_mulvey{at}hc-sc.gc.ca.

{dagger} Contributing members of the Canadian Nosocomial Infection Surveillance Program are listed in Acknowledgments.

Antimicrobial Agents and Chemotherapy, October 2004, p. 3758-3764, Vol. 48, No. 10
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.10.3758-3764.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sidjabat, H. E., Paterson, D. L., Adams-Haduch, J. M., Ewan, L., Pasculle, A. W., Muto, C. A., Tian, G.-B., Doi, Y. (2009). Molecular Epidemiology of CTX-M-Producing Escherichia coli Isolates at a Tertiary Medical Center in Western Pennsylvania. Antimicrob. Agents Chemother. 53: 4733-4739 [Abstract] [Full Text]  
  • Strahilevitz, J., Jacoby, G. A., Hooper, D. C., Robicsek, A. (2009). Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat. Clin. Microbiol. Rev. 22: 664-689 [Abstract] [Full Text]  
  • Woodford, N., Carattoli, A., Karisik, E., Underwood, A., Ellington, M. J., Livermore, D. M. (2009). Complete Nucleotide Sequences of Plasmids pEK204, pEK499, and pEK516, Encoding CTX-M Enzymes in Three Major Escherichia coli Lineages from the United Kingdom, All Belonging to the International O25:H4-ST131 Clone. Antimicrob. Agents Chemother. 53: 4472-4482 [Abstract] [Full Text]  
  • Hrabak, J., Empel, J., Bergerova, T., Fajfrlik, K., Urbaskova, P., Kern-Zdanowicz, I., Hryniewicz, W., Gniadkowski, M. (2009). International Clones of Klebsiella pneumoniae and Escherichia coli with Extended-Spectrum {beta}-Lactamases in a Czech Hospital. J. Clin. Microbiol. 47: 3353-3357 [Abstract] [Full Text]  
  • Ruppe, E., Woerther, P.-L., Diop, A., Sene, A.-M., Da Costa, A., Arlet, G., Andremont, A., Rouveix, B. (2009). Carriage of CTX-M-15-Producing Escherichia coli Isolates among Children Living in a Remote Village in Senegal. Antimicrob. Agents Chemother. 53: 3135-3137 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Campbell, L., Laupland, K. B. (2009). Molecular Characteristics of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Isolates Causing Bacteremia in the Calgary Health Region from 2000 to 2007: Emergence of Clone ST131 as a Cause of Community-Acquired Infections. Antimicrob. Agents Chemother. 53: 2846-2851 [Abstract] [Full Text]  
  • Carattoli, A. (2009). Resistance Plasmid Families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53: 2227-2238 [Full Text]  
  • Sjolund-Karlsson, M., Folster, J. P., Pecic, G., Joyce, K., Medalla, F., Rickert, R., Whichard, J. M. (2009). Emergence of Plasmid-Mediated Quinolone Resistance among Non-Typhi Salmonella enterica Isolates from Humans in the United States. Antimicrob. Agents Chemother. 53: 2142-2144 [Abstract] [Full Text]  
  • Literacka, E., Bedenic, B., Baraniak, A., Fiett, J., Tonkic, M., Jajic-Bencic, I., Gniadkowski, M. (2009). blaCTX-M Genes in Escherichia coli Strains from Croatian Hospitals Are Located in New (blaCTX-M-3a) and Widely Spread (blaCTX-M-3a and blaCTX-M-15) Genetic Structures. Antimicrob. Agents Chemother. 53: 1630-1635 [Abstract] [Full Text]  
  • Marcade, G., Deschamps, C., Boyd, A., Gautier, V., Picard, B., Branger, C., Denamur, E., Arlet, G. (2009). Replicon typing of plasmids in Escherichia coli producing extended-spectrum {beta}-lactamases. J Antimicrob Chemother 63: 67-71 [Abstract] [Full Text]  
  • Pomba, C., da Fonseca, J. D., Baptista, B. C., Correia, J. D., Martinez-Martinez, L. (2009). Detection of the Pandemic O25-ST131 Human Virulent Escherichia coli CTX-M-15-Producing Clone Harboring the qnrB2 and aac(6')-Ib-cr Genes in a Dog. Antimicrob. Agents Chemother. 53: 327-328 [Full Text]  
  • Jones, G. Ll., Warren, R. E., Skidmore, S. J., Davies, V. A., Gibreel, T., Upton, M. (2008). Prevalence and distribution of plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli lacking extended-spectrum {beta}-lactamases. J Antimicrob Chemother 62: 1245-1251 [Abstract] [Full Text]  
  • Zong, Z., Partridge, S. R., Thomas, L., Iredell, J. R. (2008). Dominance of blaCTX-M within an Australian Extended-Spectrum {beta}-Lactamase Gene Pool. Antimicrob. Agents Chemother. 52: 4198-4202 [Abstract] [Full Text]  
  • Lascols, C., Podglajen, I., Verdet, C., Gautier, V., Gutmann, L., Soussy, C.-J., Collatz, E., Cambau, E. (2008). A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae. J. Bacteriol. 190: 5217-5223 [Abstract] [Full Text]  
  • Pitout, J. D. D., Wei, Y., Church, D. L., Gregson, D. B. (2008). Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr. J Antimicrob Chemother 61: 999-1002 [Abstract] [Full Text]  
  • Baudry, P. J., Nichol, K., DeCorby, M., Mataseje, L., Mulvey, M. R., Hoban, D. J., Zhanel, G. G. (2008). Comparison of Antimicrobial Resistance Profiles among Extended-Spectrum-{beta}-Lactamase-Producing and Acquired AmpC {beta}-Lactamase-Producing Escherichia coli Isolates from Canadian Intensive Care Units. Antimicrob. Agents Chemother. 52: 1846-1849 [Abstract] [Full Text]  
  • Cordeiro, N. F., Robino, L., Medina, J., Seija, V., Bado, I., Garcia, V., Berro, M., Pontet, J., Lopez, L., Bazet, C., Rieppi, G., Gutkind, G., Ayala, J. A., Vignoli, R. (2008). Ciprofloxacin-Resistant Enterobacteria Harboring the aac(6')-Ib-cr Variant Isolated from Feces of Inpatients in an Intensive Care Unit in Uruguay. Antimicrob. Agents Chemother. 52: 806-807 [Full Text]  
  • Fang, H., Ataker, F., Hedin, G., Dornbusch, K. (2008). Molecular Epidemiology of Extended-Spectrum {beta}-Lactamases among Escherichia coli Isolates Collected in a Swedish Hospital and Its Associated Health Care Facilities from 2001 to 2006. J. Clin. Microbiol. 46: 707-712 [Abstract] [Full Text]  
  • Carattoli, A., Garcia-Fernandez, A., Varesi, P., Fortini, D., Gerardi, S., Penni, A., Mancini, C., Giordano, A. (2008). Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum -Lactamases Isolated in Rome, Italy. J. Clin. Microbiol. 46: 103-108 [Abstract] [Full Text]  
  • Fernandez, A. G., Cloeckaert, A., Bertini, A., Praud, K., Doublet, B., Weill, F.-X., Carattoli, A. (2007). Comparative Analysis of IncHI2 Plasmids Carrying blaCTX-M-2 or blaCTX-M-9 from Escherichia coli and Salmonella enterica Strains Isolated from Poultry and Humans. Antimicrob. Agents Chemother. 51: 4177-4180 [Abstract] [Full Text]  
  • Arpin, C., Coulange, L., Dubois, V., Andre, C., Fischer, I., Fourmaux, S., Grobost, F., Jullin, J., Dutilh, B., Couture, J.-F., Noury, P., Lagrange, I., Ducastaing, A., Doermann, H.-P., Quentin, C. (2007). Extended-Spectrum-{beta}-Lactamase-Producing Enterobacteriaceae Strains in Various Types of Private Health Care Centers. Antimicrob. Agents Chemother. 51: 3440-3444 [Abstract] [Full Text]  
  • Nwaneshiudu, A. I., Mucci, T., Pickard, D. J., Okeke, I. N. (2007). A Second Large Plasmid Encodes Conjugative Transfer and Antimicrobial Resistance in O119:H2 and Some Typical O111 Enteropathogenic Escherichia coli Strains. J. Bacteriol. 189: 6074-6079 [Abstract] [Full Text]  
  • Pallecchi, L., Bartoloni, A., Fiorelli, C., Mantella, A., Di Maggio, T., Gamboa, H., Gotuzzo, E., Kronvall, G., Paradisi, F., Rossolini, G. M. (2007). Rapid Dissemination and Diversity of CTX-M Extended-Spectrum {beta}-Lactamase Genes in Commensal Escherichia coli Isolates from Healthy Children from Low-Resource Settings in Latin America. Antimicrob. Agents Chemother. 51: 2720-2725 [Abstract] [Full Text]  
  • Partridge, S. R., Arlet, G. (2007). Genetic Environment of ISEcp1 and blaACC-1. Antimicrob. Agents Chemother. 51: 2658-2659 [Full Text]  
  • Mendonca, N., Leitao, J., Manageiro, V., Ferreira, E., the Antimicrobial Resistance Surveillance Program, , Canica, M. (2007). Spread of Extended-Spectrum {beta}-Lactamase CTX-M-Producing Escherichia coli Clinical Isolates in Community and Nosocomial Environments in Portugal. Antimicrob. Agents Chemother. 51: 1946-1955 [Abstract] [Full Text]  
  • Pitout, J. D. D., Church, D. L., Gregson, D. B., Chow, B. L., McCracken, M., Mulvey, M. R., Laupland, K. B. (2007). Molecular Epidemiology of CTX-M-Producing Escherichia coli in the Calgary Health Region: Emergence of CTX-M-15-Producing Isolates. Antimicrob. Agents Chemother. 51: 1281-1286 [Abstract] [Full Text]  
  • Ensor, V. M., Livermore, D. M., Hawkey, P. M. (2007). A novel reverse-line hybridization assay for identifying genotypes of CTX-M-type extended-spectrum {beta}-lactamases. J Antimicrob Chemother 59: 387-395 [Abstract] [Full Text]  
  • Novais, A., Canton, R., Moreira, R., Peixe, L., Baquero, F., Coque, T. M. (2007). Emergence and Dissemination of Enterobacteriaceae Isolates Producing CTX-M-1-Like Enzymes in Spain Are Associated with IncFII (CTX-M-15) and Broad-Host-Range (CTX-M-1, -3, and -32) Plasmids. Antimicrob. Agents Chemother. 51: 796-799 [Abstract] [Full Text]  
  • Livermore, D. M., Canton, R., Gniadkowski, M., Nordmann, P., Rossolini, G. M., Arlet, G., Ayala, J., Coque, T. M., Kern-Zdanowicz, I., Luzzaro, F., Poirel, L., Woodford, N. (2007). CTX-M: changing the face of ESBLs in Europe. J Antimicrob Chemother 59: 165-174 [Abstract] [Full Text]  
  • Tofteland, S., Haldorsen, B., Dahl, K. H., Simonsen, G. S., Steinbakk, M., Walsh, T. R., Sundsfjord, A., the Norwegian ESBL Study Group, (2007). Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-{beta}-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway. J. Clin. Microbiol. 45: 199-205 [Abstract] [Full Text]  
  • Soge, O. O., Adeniyi, B. A., Roberts, M. C. (2006). New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae. J Antimicrob Chemother 58: 1048-1053 [Abstract] [Full Text]  
  • Mamlouk, K., Boutiba-Ben Boubaker, I., Gautier, V., Vimont, S., Picard, B., Ben Redjeb, S., Arlet, G. (2006). Emergence and Outbreaks of CTX-M {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains in a Tunisian Hospital. J. Clin. Microbiol. 44: 4049-4056 [Abstract] [Full Text]  
  • Hopkins, K. L., Liebana, E., Villa, L., Batchelor, M., Threlfall, E. J., Carattoli, A. (2006). Replicon Typing of Plasmids Carrying CTX-M or CMY {beta}-Lactamases Circulating among Salmonella and Escherichia coli Isolates.. Antimicrob. Agents Chemother. 50: 3203-3206 [Abstract] [Full Text]  
  • Machado, E., Coque, T. M., Canton, R., Baquero, F., Sousa, J. C., Peixe, L., The Portuguese Resistance Study Group, (2006). Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-Producing Enterobacteriaceae Strains Containing the aac(6')-Ib-cr Gene, Which Encodes an Aminoglycoside- and Fluoroquinolone-Modifying Enzyme.. Antimicrob. Agents Chemother. 50: 3220-3221 [Full Text]  
  • Karisik, E., Ellington, M. J., Pike, R., Warren, R. E., Livermore, D. M., Woodford, N. (2006). Molecular characterization of plasmids encoding CTX-M-15 {beta}-lactamases from Escherichia coli strains in the United Kingdom. J Antimicrob Chemother 58: 665-668 [Abstract] [Full Text]  
  • Novais, A., Canton, R., Valverde, A., Machado, E., Galan, J.-C., Peixe, L., Carattoli, A., Baquero, F., Coque, T. M. (2006). Dissemination and Persistence of blaCTX-M-9 Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn402 Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-{alpha}, and IncFI Groups.. Antimicrob. Agents Chemother. 50: 2741-2750 [Abstract] [Full Text]  
  • Lavollay, M., Mamlouk, K., Frank, T., Akpabie, A., Burghoffer, B., Ben Redjeb, S., Bercion, R., Gautier, V., Arlet, G. (2006). Clonal Dissemination of a CTX-M-15 {beta}-Lactamase-Producing Escherichia coli Strain in the Paris Area, Tunis, and Bangui.. Antimicrob. Agents Chemother. 50: 2433-2438 [Abstract] [Full Text]  
  • Oteo, J., Navarro, C., Cercenado, E., Delgado-Iribarren, A., Wilhelmi, I., Orden, B., Garcia, C., Miguelanez, S., Perez-Vazquez, M., Garcia-Cobos, S., Aracil, B., Bautista, V., Campos, J. (2006). Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions.. J. Clin. Microbiol. 44: 2359-2366 [Abstract] [Full Text]  
  • Boyd, D. A., Mulvey, M. R. (2006). OXA-1 is OXA-30 is OXA-1. J Antimicrob Chemother 58: 224-225 [Full Text]  
  • Lagace-Wiens, P. R. S., Nichol, K. A., Nicolle, L. E., DeCorby, M., McCracken, M., Mulvey, M. R., Zhanel, G. G. (2006). Treatment of lower urinary tract infection caused by multidrug-resistant extended-spectrum-{beta}-lactamase-producing Escherichia coli with amoxicillin/clavulanate: case report and characterization of the isolate. J Antimicrob Chemother 57: 1262-1263 [Full Text]  
  • Mendonca, N., Louro, D., Castro, A. P., Diogo, J., Canica, M. (2006). CTX-M-15, OXA-30 and TEM-1-producing Escherichia coli in two Portuguese regions. J Antimicrob Chemother 57: 1014-1016 [Full Text]  
  • Shibata, N., Kurokawa, H., Doi, Y., Yagi, T., Yamane, K., Wachino, J.-i., Suzuki, S., Kimura, K., Ishikawa, S., Kato, H., Ozawa, Y., Shibayama, K., Kai, K., Konda, T., Arakawa, Y. (2006). PCR Classification of CTX-M-Type {beta}-Lactamase Genes Identified in Clinically Isolated Gram-Negative Bacilli in Japan. Antimicrob. Agents Chemother. 50: 791-795 [Abstract] [Full Text]  
  • Wachino, J.-i., Yamane, K., Shibayama, K., Kurokawa, H., Shibata, N., Suzuki, S., Doi, Y., Kimura, K., Ike, Y., Arakawa, Y. (2006). Novel Plasmid-Mediated 16S rRNA Methylase, RmtC, Found in a Proteus mirabilis Isolate Demonstrating Extraordinary High-Level Resistance against Various Aminoglycosides. Antimicrob. Agents Chemother. 50: 178-184 [Abstract] [Full Text]  
  • Eckert, C., Gautier, V., Arlet, G. (2006). DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother 57: 14-23 [Abstract] [Full Text]  
  • Xu, L., Ensor, V., Gossain, S., Nye, K., Hawkey, P. (2005). Rapid and simple detection of blaCTX-M genes by multiplex PCR assay. J Med Microbiol 54: 1183-1187 [Abstract] [Full Text]  
  • Pitout, J. D. D., Laupland, K. B., Church, D. L., Menard, M. L., Johnson, J. R. (2005). Virulence Factors of Escherichia coli Isolates That Produce CTX-M-Type Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 49: 4667-4670 [Abstract] [Full Text]  
  • Johnson, T. J., Siek, K. E., Johnson, S. J., Nolan, L. K. (2005). DNA Sequence and Comparative Genomics of pAPEC-O2-R, an Avian Pathogenic Escherichia coli Transmissible R Plasmid. Antimicrob. Agents Chemother. 49: 4681-4688 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Church, D. L., Elsayed, S., Laupland, K. B. (2005). Community-Wide Outbreaks of Clonally Related CTX-M-14 {beta}-Lactamase-Producing Escherichia coli Strains in the Calgary Health Region. J. Clin. Microbiol. 43: 2844-2849 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»