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Antimicrobial Agents and Chemotherapy, November 2004, p. 4457-4459, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4457-4459.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of a Colorimetric Antifungal Susceptibility Test by Using 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride

Jeong Hwan Shin,1 Jae-Cheol Choi,2 Jeong Nyeo Lee,1 Hyung Hoi Kim,2 Eun Yup Lee,2 and Chulhun Ludgerus Chang2,3*

Department of Laboratory Medicine, Busan Paik Hospital, Inje University College of Medicine,1 Department of Laboratory Medicine,2 Pusan National University College of Medicine and Medical Research Institute, Pusan National University, Busan, Korea3

Received 15 January 2004/ Returned for modification 24 May 2004/ Accepted 30 June 2004

A colorimetric antifungal susceptibility test was performed using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. Among 24 strains of Candida species, no trailing growth was found. In 22 and 20 strains, the MICs obtained in the colorimetric assay were within two dilutions of those obtained by the National Committee for Clinical Laboratory Standards method for ketoconazole and itraconazole, respectively.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Pusan National University College of Medicine, Seo-Gu Ami-Dong 1-Ga 10, Busan 602-739, Korea. Phone: 82-51-240-7418. Fax: 82-51-247-6560. E-mail: cchl{at}pusan.ac.kr.

Antimicrobial Agents and Chemotherapy, November 2004, p. 4457-4459, Vol. 48, No. 11
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.11.4457-4459.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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