This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pallecchi, L.
Right arrow Articles by Rossolini, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pallecchi, L.
Right arrow Articles by Rossolini, G. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 2004, p. 4556-4561, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4556-4561.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of CTX-M-Type ß-Lactamase Genes in Fecal Escherichia coli Isolates from Healthy Children in Bolivia and Peru

Lucia Pallecchi,1 Monica Malossi,1 Antonia Mantella,2 Eduardo Gotuzzo,3 Christian Trigoso,4 Alessandro Bartoloni,2 Franco Paradisi,2 Göran Kronvall,5 and Gian Maria Rossolini1*

Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena,1 Clinica delle Malattie Infettive, Dipartimento di Area Critica Medico-Chirurgica, Università di Firenze, Florence, Italy,2 Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru,3 Instituto Nacional de Laboratorios de Salud INLASA, La Paz, Bolivia,4 Department of Microbiology and Tumor Biology-MTC, Clinical Microbiology, Karolinska Institute, Karolinska Hospital L2:02, Stockholm, Sweden5

Received 18 March 2004/ Returned for modification 31 May 2004/ Accepted 30 July 2004

A survey was carried out from August to November 2002 to evaluate the antimicrobial susceptibilities of fecal Escherichia coli isolates from 3,208 healthy children from four different urban areas of Latin America, two in Bolivia (Camiri and Villa Montes) and two in Peru (Yurimaguas and Moyobamba). Ceftriaxone-resistant E. coli isolates were detected in four children, one from each of the areas sampled. The isolates exhibited a multidrug-resistant phenotype, including resistance to oxyimino-cephalosporins and aztreonam, and the MICs of ceftazidime for the isolates were lower than those of cefotaxime. By PCR and sequencing, the blaCTX-M-2 determinant was detected in three isolates and the blaCTX-M-15 determinant was detected in one isolate (from Peru). The CTX-M-2-producing isolates belonged to three different phylogenetic groups (groups A, B2, and D), while the CTX-M-15-producing isolate belonged to phylogenetic group D. The blaCTX-M-2 determinants were transferable to E. coli by conjugation, while conjugative transfer of the blaCTX-M-15 determinant was not detectable. Plasmids harboring the blaCTX-M-2 determinant exhibited similar restriction profiles, and in all of them the gene was located on a 2.2-kb PstI fragment, suggesting a genetic environment similar to that present in In35 and InS21. The findings of the present study confirm the widespread distribution of CTX-M-type ß-lactamases and underscore the role that commensal E. coli isolates could play as a potential reservoir of these clinically relevant resistance determinants. This is the first report of CTX-M-type enzymes in Bolivia and Peru and also the first report of the detection of CTX-M-15 in Latin America.

* Corresponding author. Mailing address: Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Policlinico "Le Scotte," 53100 Siena, Italy. Phone: 39 0577 233327. Fax: 39 0577 233325. E-mail: rossolini{at}unisi.it.

Antimicrobial Agents and Chemotherapy, December 2004, p. 4556-4561, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4556-4561.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ruppe, E., Woerther, P.-L., Diop, A., Sene, A.-M., Da Costa, A., Arlet, G., Andremont, A., Rouveix, B. (2009). Carriage of CTX-M-15-Producing Escherichia coli Isolates among Children Living in a Remote Village in Senegal. Antimicrob. Agents Chemother. 53: 3135-3137 [Abstract] [Full Text]  
  • Leflon-Guibout, V., Blanco, J., Amaqdouf, K., Mora, A., Guize, L., Nicolas-Chanoine, M.-H. (2008). Absence of CTX-M Enzymes but High Prevalence of Clones, Including Clone ST131, among Fecal Escherichia coli Isolates from Healthy Subjects Living in the Area of Paris, France. J. Clin. Microbiol. 46: 3900-3905 [Abstract] [Full Text]  
  • Nicolas-Chanoine, M.-H., Blanco, J., Leflon-Guibout, V., Demarty, R., Alonso, M. P., Canica, M. M., Park, Y.-J., Lavigne, J.-P., Pitout, J., Johnson, J. R. (2008). Intercontinental emergence of Escherichia coli clone O25:H4-ST131 producing CTX-M-15. J Antimicrob Chemother 61: 273-281 [Abstract] [Full Text]  
  • Walk, S. T., Mladonicky, J. M., Middleton, J. A., Heidt, A. J., Cunningham, J. R., Bartlett, P., Sato, K., Whittam, T. S. (2007). Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms. Appl. Environ. Microbiol. 73: 5982-5989 [Abstract] [Full Text]  
  • Pallecchi, L., Bartoloni, A., Fiorelli, C., Mantella, A., Di Maggio, T., Gamboa, H., Gotuzzo, E., Kronvall, G., Paradisi, F., Rossolini, G. M. (2007). Rapid Dissemination and Diversity of CTX-M Extended-Spectrum {beta}-Lactamase Genes in Commensal Escherichia coli Isolates from Healthy Children from Low-Resource Settings in Latin America. Antimicrob. Agents Chemother. 51: 2720-2725 [Abstract] [Full Text]  
  • Mendonca, N., Leitao, J., Manageiro, V., Ferreira, E., the Antimicrobial Resistance Surveillance Program, , Canica, M. (2007). Spread of Extended-Spectrum {beta}-Lactamase CTX-M-Producing Escherichia coli Clinical Isolates in Community and Nosocomial Environments in Portugal. Antimicrob. Agents Chemother. 51: 1946-1955 [Abstract] [Full Text]  
  • Pleydell, E. J., Brown, P. E., Woodward, M. J., Davies, R. H., French, N. P. (2007). Sources of Variation in the Ampicillin-Resistant Escherichia coli Concentration in the Feces of Organic Broiler Chickens. Appl. Environ. Microbiol. 73: 203-210 [Abstract] [Full Text]  
  • Celenza, G., Pellegrini, C., Caccamo, M., Segatore, B., Amicosante, G., Perilli, M. (2006). Spread of blaCTX-M-type and blaPER-2 {beta}-lactamase genes in clinical isolates from Bolivian hospitals. J Antimicrob Chemother 57: 975-978 [Abstract] [Full Text]  
  • Eckert, C., Gautier, V., Arlet, G. (2006). DNA sequence analysis of the genetic environment of various blaCTX-M genes. J Antimicrob Chemother 57: 14-23 [Abstract] [Full Text]  
  • Mugnaioli, C., Luzzaro, F., De Luca, F., Brigante, G., Amicosante, G., Rossolini, G. M. (2005). Dissemination of CTX-M-Type Extended-Spectrum {beta}-Lactamase Genes to Unusual Hosts. J. Clin. Microbiol. 43: 4183-4185 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»