This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mendes, R. E.
Right arrow Articles by Walsh, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mendes, R. E.
Right arrow Articles by Walsh, T. R.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 2004, p. 4693-4702, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4693-4702.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Integron Carrying a Novel Metallo-ß-Lactamase Gene, blaIMP-16, and a Fused Form of Aminoglycoside-Resistant Gene aac(6')-30/aac(6')-Ib': Report from the SENTRY Antimicrobial Surveillance Program

Rodrigo E. Mendes,1,2* Mark A. Toleman,2 Julival Ribeiro,3 Helio S. Sader,1,4 Ronald N. Jones,4,5 and Timothy R. Walsh2

Disciplina de Doenças Infecciosas e Parasitárias, Universidade Federal de São Paulo, São Paulo,1 Hospital de Base do Distrito Federal, Brasília, Brazil,3 Department of Pathology & Microbiology, University of Bristol, Bristol, United Kingdom,2 The JONES Group/JMI Laboratories, North Liberty, Iowa,4 Tufts University School of Medicine, Boston, Massachusetts5

Received 19 April 2004/ Returned for modification 26 June 2004/ Accepted 4 September 2004

Since January 2002 Pseudomonas sp. strains resistant to carbapenems and ceftazidime have been routinely screened as part of the SENTRY Antimicrobial Surveillance Program for metallo-ß-lactamase production, and their resistance determinants have been analyzed. Pseudomonas aeruginosa index strain 101-4704, which harbors a novel blaIMP variant, blaIMP-16, was isolated in April 2002 from a 60-year-old man in Brasília, Brazil. blaIMP-16 was found on the chromosome of the P. aeruginosa index strain, and the deduced amino acid sequence (IMP-16) showed the greatest identities to IMP-11 (90.3%) and IMP-8 (89.5%). Sequence analysis revealed that blaIMP-16 was associated with a class 1 integron, which also encoded aminoglycoside-modifying enzymes. Downstream of blaIMP-16 resided an open reading frame, which consisted of a new aminoglycoside-modifying gene, namely, aac(6')-30, which was fused with aac(6')-Ib'. The amino acid sequence of the aac(6')-30 putative protein showed the most identity (52.7%) to the sequence of AAC(6')-29b described previously. The fourth gene cassette constituted aadA1. The steady-state kinetics of IMP-16 demonstrated that the enzyme preferred cephalosporins and carbapenems to penicillins. The main functional difference observed among the kinetic values for IMP-16 compared to those for other IMPs was a lack of cefoxitin hydrolysis and a lower kcat/Km value for imipenem (0.36 µM–1 · s–1). This report further emphasizes the spread of metallo-ß-lactamase genes and their close association with various aminoglycoside resistance genes.

* Corresponding author. Mailing address: Laboratório Especial de Microbiologia Clínica, Division of Infectious Diseases, Universidade Federal de São Paulo, Rua Leandro Dupret, 188 São Paulo, SP 04025-010, Brazil. Phone: (55-11) 5081-2819. Fax: (55-11) 5571-5180. E-mail: rodrigo.mendes{at}lemc.com.br.

Antimicrobial Agents and Chemotherapy, December 2004, p. 4693-4702, Vol. 48, No. 12
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.12.4693-4702.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • al Naiemi, N., Rijnsburger, M. C., Savelkoul, P. H. M., Vandenbroucke-Grauls, C. M. J., Debets-Ossenkopp, Y. J. (2009). Emergence of metallo-{beta}-lactamases in the Netherlands. J Med Microbiol 58: 1398-1399 [Full Text]  
  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Pellegrini, C., Mercuri, P. S., Celenza, G., Galleni, M., Segatore, B., Sacchetti, E., Volpe, R., Amicosante, G., Perilli, M. (2009). Identification of blaIMP-22 in Pseudomonas spp. in urban wastewater and nosocomial environments: biochemical characterization of a new IMP metallo-enzyme variant and its genetic location. J Antimicrob Chemother 63: 901-908 [Abstract] [Full Text]  
  • Juan, C., Beceiro, A., Gutierrez, O., Alberti, S., Garau, M., Perez, J. L., Bou, G., Oliver, A. (2008). Characterization of the New Metallo-{beta}-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain. Antimicrob. Agents Chemother. 52: 3589-3596 [Abstract] [Full Text]  
  • Marchiaro, P., Ballerini, V., Spalding, T., Cera, G., Mussi, M. A., Moran-Barrio, J., Vila, A. J., Viale, A. M., Limansky, A. S. (2008). A convenient microbiological assay employing cell-free extracts for the rapid characterization of Gram-negative carbapenemase producers. J Antimicrob Chemother 62: 336-344 [Abstract] [Full Text]  
  • Picao, R. C., Andrade, S. S., Nicoletti, A. G., Campana, E. H., Moraes, G. C., Mendes, R. E., Gales, A. C. (2008). Metallo-{beta}-Lactamase Detection: Comparative Evaluation of Double-Disk Synergy versus Combined Disk Tests for IMP-, GIM-, SIM-, SPM-, or VIM-Producing Isolates. J. Clin. Microbiol. 46: 2028-2037 [Abstract] [Full Text]  
  • Mendes, R. E., Bell, J. M., Turnidge, J. D., Yang, Q., Yu, Y., Sun, Z., Jones, R. N. (2008). Carbapenem-Resistant Isolates of Klebsiella pneumoniae in China and Detection of a Conjugative Plasmid (blaKPC-2 plus qnrB4) and a blaIMP-4 Gene. Antimicrob. Agents Chemother. 52: 798-799 [Full Text]  
  • Mendes, R. E., Castanheira, M., Toleman, M. A., Sader, H. S., Jones, R. N., Walsh, T. R. (2007). Characterization of an Integron Carrying blaIMP-1 and a New Aminoglycoside Resistance Gene, aac(6')-31, and Its Dissemination among Genetically Unrelated Clinical Isolates in a Brazilian Hospital. Antimicrob. Agents Chemother. 51: 2611-2614 [Abstract] [Full Text]  
  • Mendes, R. E., Kiyota, K. A., Monteiro, J., Castanheira, M., Andrade, S. S., Gales, A. C., Pignatari, A. C. C., Tufik, S. (2007). Rapid Detection and Identification of Metallo-{beta}-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis. J. Clin. Microbiol. 45: 544-547 [Abstract] [Full Text]  
  • Marra, A. R., Pereira, C. A. P., Gales, A. C., Menezes, L. C., Cal, R. G. R., de Souza, J. M. A., Edmond, M. B., Faro, C., Wey, S. B. (2006). Bloodstream Infections with Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa: Epidemiology, Microbiology, and Clinical Outcomes. Antimicrob. Agents Chemother. 50: 388-390 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»