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Antimicrobial Agents and Chemotherapy, April 2004, p. 1426-1429, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1426-1429.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Field Evaluation of a Novel Colorimetric Method—Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay—To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

Alan Brockman,1,2,3* Sittaporn Singlam,1,2 Lucy Phiaphun,1 Sornchai Looareesuwan,2 Nicholas J. White,2,4 and François Nosten1,2,4

Shoklo Malaria Research Unit, Mae Sot, Tak 63110,1 Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand,2 Australian Centre for International Tropical Health and Nutrition, University of Queensland, Brisbane, Australia,3 Centre for Tropical Diseases, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom4

Received 22 August 2003/ Returned for modification 2 November 2003/ Accepted 27 December 2003

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/µl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

* Corresponding author. Present address: International Health Program, Menzies School of Health Research, P.O. Box 41096, Casuarina, Northern Territory 0810, Australia. Phone: 61-8-89228197. Fax: 61-8-89278888. E-mail: alan.brockman{at}menzies.edu.au.

Antimicrobial Agents and Chemotherapy, April 2004, p. 1426-1429, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1426-1429.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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