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Antimicrobial Agents and Chemotherapy, May 2004, p. 1908-1911, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1908-1911.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Inhalational Murine Model of Invasive Pulmonary Aspergillosis

Donald C. Sheppard,1 Gunter Rieg,1 Lisa Y. Chiang,1 Scott G. Filler,1,2 John E. Edwards Jr.,1,2 and Ashraf S. Ibrahim1,2*

St. John's Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 90502,1 The David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 900242

Received 4 December 2003/ Returned for modification 31 December 2003/ Accepted 15 January 2004

We developed a novel model of invasive aspergillosis (IA) that recapitulates human disease. Mice were immunosuppressed with cyclophosphamide and cortisone acetate and then infected in an aerosol chamber. This procedure reproducibly delivered 1 x 103 to 3 x 103 conidia to the lungs. Lethal pulmonary IA developed over 2 weeks and was prevented by amphotericin B.

* Corresponding author. Mailing address: Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, 1124 W. Carson St., Torrance, CA 90502. Phone: (310) 222-3813. Fax: (310) 782-2016. E-mail: ibrahim{at}humc.edu.

Antimicrobial Agents and Chemotherapy, May 2004, p. 1908-1911, Vol. 48, No. 5
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.5.1908-1911.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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