This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bronstein, J. C.
Right arrow Articles by Weber, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bronstein, J. C.
Right arrow Articles by Weber, P. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2004, p. 2708-2711, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2708-2711.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Purification and Characterization of Recombinant Staphylococcus haemolyticus DNA Gyrase and Topoisomerase IV Expressed in Escherichia coli

Joel C. Bronstein,1* Stacey L. Olson,2 Kristin LeVier,1 Mark Tomilo,3 and Peter C. Weber1

Antibacterial Molecular Sciences and Technologies,1 Antibacterial Pharmacology,3 Manpower Professional, Pfizer Global Research and Development, Ann Arbor, Michigan 481052

Received 3 September 2003/ Returned for modification 22 November 2003/ Accepted 31 March 2004

The subunits of DNA gyrase and topoisomerase IV from Staphylococcus haemolyticus were expressed in Escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. This represents the first description of a method for isolating type II topoisomerases of a coagulase-negative staphylococcal species.

* Corresponding author. Mailing address: Pfizer Global Research and Development, 2800 Plymouth Rd., Ann Arbor, MI 48105. Phone: (734) 622-7682. Fax: (734) 622-7158. E-mail: Joel.Bronstein{at}pfizer.com.

Antimicrobial Agents and Chemotherapy, July 2004, p. 2708-2711, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2708-2711.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»