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Antimicrobial Agents and Chemotherapy, July 2004, p. 2736-2738, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2736-2738.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Isolates in Portugal

Isabel Portugal,1* Luís Barreiro,2 José Moniz-Pereira,1 and Laura Brum2

Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa,1 Unidade de Micobactérias, Centro de Bacteriologia, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisbon, Portugal2

Received 3 November 2003/ Returned for modification 16 December 2003/ Accepted 15 March 2004

The nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were pyrazinamidase (PZase) negative. Four strains contained a wild-type pncA gene, and PZase activity was undetectable in two of these strains. Seven of the 18 identified pncA mutations found have not been described in previous studies.

* Corresponding author. Mailing address: Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Rua Prof. Gama Pinto, 1649-003 Lisbon, Portugal. Phone: (351) 21 7946439. Fax: (351) 21 7934212. E-mail: isabel.portugal{at}ff.ul.pt.

Antimicrobial Agents and Chemotherapy, July 2004, p. 2736-2738, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2736-2738.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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