This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ahmed, A. O. A. Articles by Bakker-Woudenberg, I. A. J. M. Search for Related Content PubMed PubMed Citation Articles by Ahmed, A. O. A. Articles by Bakker-Woudenberg, I. A. J. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, July 2004, p. 2742-2746, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2742-2746.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay

Abdalla O. A. Ahmed,¹ Wendy W. J. van de Sande,² Wim van Vianen,² Alex van Belkum,^2* Ahmed H. Fahal,¹ Henri A. Verbrugh,² and Irma A. J. M. Bakker-Woudenberg²

Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan,¹ Department of Medical Microbiology & Infectious Diseases, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands²

Received 10 November 2003/ Returned for modification 5 December 2003/ Accepted 27 February 2004

Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC₅₀]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC₅₀ of 0.5 to 1.0 mg/liter).

---

* Corresponding author. Mailing address: Department of Medical Microbiology and Infections Diseases, Erasmus MC University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31-10-4635813. Fax: 31-10-4633875. E-mail: a.vanbelkum{at}erasmusmc.nl.

---

Antimicrobial Agents and Chemotherapy, July 2004, p. 2742-2746, Vol. 48, No. 7
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.7.2742-2746.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• van de Sande, W. W. J., Fahal, A. H., Riley, T. V., Verbrugh, H., van Belkum, A. (2007). In vitro susceptibility of Madurella mycetomatis, prime agent of Madura foot, to tea tree oil and artemisinin. J Antimicrob Chemother 59: 553-555 [Abstract] [Full Text]  
• van de Sande, W. W. J., Janse, D.-J., Hira, V., Goedhart, H., van der Zee, R., Ahmed, A. O. A., Ott, A., Verbrugh, H., van Belkum, A. (2006). Translationally Controlled Tumor Protein from Madurella mycetomatis, a Marker for Tumorous Mycetoma Progression. J. Immunol. 177: 1997-2005 [Abstract] [Full Text]  
• Antachopoulos, C., Meletiadis, J., Roilides, E., Sein, T., Walsh, T. J. (2006). Rapid Susceptibility Testing of Medically Important Zygomycetes by XTT Assay. J. Clin. Microbiol. 44: 553-560 [Abstract] [Full Text]  
• van de Sande, W. W. J., Luijendijk, A., Ahmed, A. O. A., Bakker-Woudenberg, I. A. J. M., van Belkum, A. (2005). Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method. Antimicrob. Agents Chemother. 49: 1364-1368 [Abstract] [Full Text]