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Antimicrobial Agents and Chemotherapy, August 2004, p. 3147-3150, Vol. 48, No. 8
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.8.3147-3150.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of pH on the In Vitro Activities of Amphotericin B, Itraconazole, and Flucytosine against Aspergillus Isolates

D. T. A. Te Dorsthorst,1,2,3 J. W. Mouton,1 C. J. P. van den Beukel,2,3 H. A. L. van der Lee,2,3 J. F. G. M. Meis,1 and P. E. Verweij2,3*

Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital,1 Department of Medical Microbiology, University Medical Center,2 Nijmegen University Center for Infectious Diseases, Nijmegen, The Netherlands3

Received 23 December 2003/ Returned for modification 17 February 2004/ Accepted 24 April 2004

The in vitro susceptibilities of 21 Aspergillus isolates were tested against three antifungal agents in RPMI 1640 and yeast nitrogen base at pH 5.0 and 7.0 by a broth microdilution format of the NCCLS method. The MICs of amphotericin B and itraconazole were higher, while those of flucytosine were lower, at pH 5.0 than at pH 7.0. The poor correlation between in vitro results and clinical outcome could be due to a difference in pH between the in vitro susceptibility test and at the site of infection.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Medical Center Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. Phone: 31-(0)24-3614356. Fax: 31-(0)24-3540216. E-mail: p.verweij{at}mmb.umcn.nl.

Antimicrobial Agents and Chemotherapy, August 2004, p. 3147-3150, Vol. 48, No. 8
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.8.3147-3150.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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