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Antimicrobial Agents and Chemotherapy, September 2004, p. 3613-3617, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3613-3617.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Local Genetic Patterns within a Vancomycin-Resistant Enterococcus faecalis Clone Isolated in Three Hospitals in Portugal

Carla Novais,1 Teresa M. Coque,2 João Carlos Sousa,1 Fernando Baquero,2 Luisa Peixe,1* and the Portuguese Resistance Study Group{dagger}

Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal,1 Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Madrid, Spain2

Received 16 January 2004/ Returned for modification 29 February 2004/ Accepted 18 May 2004

Eight pulsed-field gel electrophoresis subtypes and six Tn1546 variants were identified among Enterococcus faecalis isolates of a single clone recovered in three geographically separate Portuguese hospitals. Some clonal subtypes were found in particular hospitals, and Tn1546 variants were either widespread or confined to some of them. We also report on the first Tn1546 transposon containing an ISEf1 insertion.

* Corresponding author. Mailing address: Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 4050 Porto, Portugal. Phone: 351-2-22078946. Fax: 351-2-2003977. E-mail: lpeixe{at}ff.up.pt.

{dagger} Contributing members of the Portuguese Resistance Study Group are listed in Acknowledgments.

Antimicrobial Agents and Chemotherapy, September 2004, p. 3613-3617, Vol. 48, No. 9
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.9.3613-3617.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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