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Antimicrobial Agents and Chemotherapy, July 2005, p. 3014-3017, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3014-3017.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of the blaCTX-M-2 Gene in Salmonella enterica Serovar Infantis

José A. Di Conza,1 Gabriel O. Gutkind,1* Marta E. Mollerach,1 and Juan A. Ayala2

Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 1113 Buenos Aires, Argentina,1 Centro de Biología Molecular "Severo Ochoa," CSIC-UAM, Campus de Cantoblanco, 28049 Madrid, Spain2

Received 5 October 2004/ Returned for modification 28 November 2004/ Accepted 16 April 2005

Transcriptional organization of blaCTX-M-2 present in a multiresistance plasmid of Salmonella enterica serovar Infantis suggests the presence of more than one promoter involved in the expression of the ß-lactamase gene. At least two blaCTX-M-2-specific mRNAs (near to 1 kb and 5 kb) were evidenced. Two +1 signals were detected at –22 bp and –59 bp of blaCTX-M-2 defining two putative promoters.

* Corresponding author. Mailing address: Cátedra de Microbiología, Facultad de Farmacia y Bioquímica (UBA), Junin 956, 8vo Piso, (1113), Capital Federal, Buenos Aires, Argentina. Phone: 54 (11) 4964-8285. Fax: 54 (11) 4964-8274. E-mail: ggutkind{at}ffyb.uba.ar.

Antimicrobial Agents and Chemotherapy, July 2005, p. 3014-3017, Vol. 49, No. 7
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.7.3014-3017.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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