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Antimicrobial Agents and Chemotherapy, August 2005, p. 3435-3441, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3435-3441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Establishment of a Superficial Skin Infection Model in Mice by Using Staphylococcus aureus and Streptococcus pyogenes

Elisabeth Kugelberg,^1,2, Tobias Norström,^3, Thomas K. Petersen,⁴ Tore Duvold,⁴ Dan I. Andersson,^1,2 and Diarmaid Hughes^3*

Department of Bacteriology, Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden,¹ Microbiology and Tumor Biology Center, Karolinska Institute, S-171 77 Stockholm, Sweden,² Department of Cell and Molecular Biology, Box 596, The Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden,³ LEO Pharma, DK-2750 Ballerup, Denmark⁴

Received 9 August 2004/ Returned for modification 18 September 2004/ Accepted 27 April 2005

A new animal model for the purpose of studying superficial infections is presented. In this model an infection is established by disruption of the skin barrier by partial removal of the epidermal layer by tape stripping and subsequent application of the pathogens Staphylococcus aureus and Streptococcus pyogenes. The infection and the infection route are purely topical, in contrast to those used in previously described animal models in mice, such as the skin suture-wound model, where the infection is introduced into the deeper layers of the skin. Thus, the present model is considered more biologically relevant for the study of superficial skin infections in mice and humans. Established topical antibiotic treatments are shown to be effective. The procedures involved in the model are simple, a feature that increases throughput and reproducibility. This new model should be applicable to the evaluation of novel antimicrobial treatments of superficial infections caused by S. aureus and S. pyogenes.

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* Corresponding author. Mailing address: Department of Cell and Molecular Biology, Box 596, The Biomedical Center, Uppsala University, S-751 24 Uppsala, Sweden. Phone: 46-18-471 4354. Fax: 48-18-530396. E-mail: diarmaid.hughes{at}icm.uu.se.

E.K. and T.N. contributed equally to this work.

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Antimicrobial Agents and Chemotherapy, August 2005, p. 3435-3441, Vol. 49, No. 8
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.8.3435-3441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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