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Antimicrobial Agents and Chemotherapy, September 2005, p. 3948-3951, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3948-3951.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of a Novel Trimethoprim-Resistant Dihydrofolate Reductase from a Nosocomial Isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

Jun-ichiro Sekiguchi,1 Prasit Tharavichitkul,3 Tohru Miyoshi-Akiyama,1 Vena Chupia,3 Tomoko Fujino,1 Minako Araake,1 Atsushi Irie,1 Koji Morita,4 Tadatoshi Kuratsuji,2,5 and Teruo Kirikae1*

Department of Infectious Diseases,1 Research Institute, International Medical Center of Japan, Shinjuku, Tokyo 162-8655, Japan,2 Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand,3 Department of Microbiology, Kyorin University School of Health Sciences, Hachioji, Tokyo 192-8508, Japan,4 National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan5

Received 16 March 2005/ Returned for modification 24 April 2005/ Accepted 14 June 2005

A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.

* Corresponding author. Mailing address: Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. Phone: (81) 3 3202 7181, ext. 2838. Fax: (81) 3 3202 7364. E-mail: tkirikae{at}ri.imcj.go.jp.

Antimicrobial Agents and Chemotherapy, September 2005, p. 3948-3951, Vol. 49, No. 9
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.9.3948-3951.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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