This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yin, S.
Right arrow Articles by Boyle-Vavra, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yin, S.
Right arrow Articles by Boyle-Vavra, S.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, January 2006, p. 336-343, Vol. 50, No. 1
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.1.336-343.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

VraSR Two-Component Regulatory System and Its Role in Induction of pbp2 and vraSR Expression by Cell Wall Antimicrobials in Staphylococcus aureus{dagger}

Shaohui Yin,1 Robert S. Daum,1,2,3 and Susan Boyle-Vavra1*

Department of Pediatrics,1 Committee on Microbiology,2 Committee on Molecular Medicine, The University of Chicago, Chicago, Illinois 606373

Received 15 June 2005/ Returned for modification 8 August 2005/ Accepted 18 October 2005

The VraS/VraR two-component system (VraSR) regulates transcriptional induction of penicillin-binding protein 2 (encoded by pbp2) by vancomycin in Staphylococcus aureus. We have now defined the vraSR operon and determined that its induction by ß-lactams as well as by vancomycin is autoregulated. Induction of the pbp2 and vraSR operons by ß-lactams and related compounds within 1 hour after exposure to the antimicrobials was dependent on vraS. However, when a disk diffusion assay that can detect induction of genes over an extended time period was used, induction of the pbp2 operon was mediated by some ß-lactams, including oxacillin; this induction was independent of vraS.

* Corresponding author. Mailing address: Department of Pediatrics, The University of Chicago, 5841 S. Maryland Ave., MC 6054, Chicago, IL 60637. Phone: (773) 702-6401. Fax: (773) 702-1196. E-mail: sboyleva{at}midway.uchicago.edu.

{dagger} Supplemental material for this article may be found at http://aac.asm.org/.

Antimicrobial Agents and Chemotherapy, January 2006, p. 336-343, Vol. 50, No. 1
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.1.336-343.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kato, Y., Suzuki, T., Ida, T., Maebashi, K. (2009). Genetic changes associated with glycopeptide resistance in Staphylococcus aureus: predominance of amino acid substitutions in YvqF/VraSR. J Antimicrob Chemother 0: dkp394v1-dkp394 [Abstract] [Full Text]  
  • Katayama, Y., Murakami-Kuroda, H., Cui, L., Hiramatsu, K. (2009). Selection of Heterogeneous Vancomycin-Intermediate Staphylococcus aureus by Imipenem. Antimicrob. Agents Chemother. 53: 3190-3196 [Abstract] [Full Text]  
  • Suntharalingam, P., Senadheera, M. D., Mair, R. W., Levesque, C. M., Cvitkovitch, D. G. (2009). The LiaFSR System Regulates the Cell Envelope Stress Response in Streptococcus mutans. J. Bacteriol. 191: 2973-2984 [Abstract] [Full Text]  
  • Cui, L., Neoh, H.-m., Shoji, M., Hiramatsu, K. (2009). Contribution of vraSR and graSR Point Mutations to Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 1231-1234 [Abstract] [Full Text]  
  • Zhang, J., Biswas, I. (2009). A phenotypic microarray analysis of a Streptococcus mutans liaS mutant. Microbiology 155: 61-68 [Abstract] [Full Text]  
  • Goldstein, F., Perutka, J., Cuirolo, A., Plata, K., Faccone, D., Morris, J., Sournia, A., Kitzis, M. D., Ly, A., Archer, G., Rosato, A. E. (2007). Identification and Phenotypic Characterization of a {beta}-Lactam-Dependent, Methicillin-Resistant Staphylococcus aureus Strain. Antimicrob. Agents Chemother. 51: 2514-2522 [Abstract] [Full Text]  
  • Mwangi, M. M., Wu, S. W., Zhou, Y., Sieradzki, K., de Lencastre, H., Richardson, P., Bruce, D., Rubin, E., Myers, E., Siggia, E. D., Tomasz, A. (2007). Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. Proc. Natl. Acad. Sci. USA 104: 9451-9456 [Abstract] [Full Text]  
  • Seggewiss, J., Becker, K., Kotte, O., Eisenacher, M., Yazdi, M. R. K., Fischer, A., McNamara, P., Al Laham, N., Proctor, R., Peters, G., Heinemann, M., von Eiff, C. (2006). Reporter Metabolite Analysis of Transcriptional Profiles of a Staphylococcus aureus Strain with Normal Phenotype and Its Isogenic hemB Mutant Displaying the Small-Colony-Variant Phenotype. J. Bacteriol. 188: 7765-7777 [Abstract] [Full Text]  
  • Wecke, T., Veith, B., Ehrenreich, A., Mascher, T. (2006). Cell Envelope Stress Response in Bacillus licheniformis: Integrating Comparative Genomics, Transcriptional Profiling, and Regulon Mining To Decipher a Complex Regulatory Network. J. Bacteriol. 188: 7500-7511 [Abstract] [Full Text]  
  • Gardete, S., Wu, S. W., Gill, S., Tomasz, A. (2006). Role of VraSR in Antibiotic Resistance and Antibiotic-Induced Stress Response in Staphylococcus aureus.. Antimicrob. Agents Chemother. 50: 3424-3434 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»