This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wachino, J.-i.
Right arrow Articles by Arakawa, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wachino, J.-i.
Right arrow Articles by Arakawa, Y.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2006, p. 534-541, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.534-541.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Horizontal Transfer of blaCMY-Bearing Plasmids among Clinical Escherichia coli and Klebsiella pneumoniae Isolates and Emergence of Cefepime-Hydrolyzing CMY-19

Jun-ichi Wachino,1,2 Hiroshi Kurokawa,1 Satowa Suzuki,1 Kunikazu Yamane,1 Naohiro Shibata,1 Kouji Kimura,1 Yasuyoshi Ike,2 and Yoshichika Arakawa1*

Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo,1 Department of Bacteriology and Bacterial Infection Control, Gunma University Graduate School of Medicine, Gunma, Japan2

Received 30 June 2005/ Returned for modification 17 August 2005/ Accepted 4 November 2005

Nine Escherichia coli and 5 Klebsiella pneumoniae clinical isolates resistant to various cephalosporins and cephamycins were identified in a Japanese general hospital between 1995 and 1997. All nine E. coli isolates and one K. pneumoniae isolate carried blaCMY-9, while the other four K. pneumoniae isolates harbored a variant of blaCMY-9, namely, blaCMY-19. The pulsed-field gel electrophoresis patterns of the nine CMY-9-producing E. coli isolates were almost identical, suggesting their clonal relatedness, while those of the five K. pneumoniae isolates were divergent. Plasmid profiles, Southern hybridization, and conjugation assays revealed that the genes for the CMY-9 and the CMY-19 ß-lactamases were located on very similar conjugative plasmids in E. coli and K. pneumoniae. The genetic environment of blaCMY-19 was identical to that of blaCMY-9. A single amino acid substitution, I292S, adjacent to the H-10 helix region was observed between CMY-9 and CMY-19. This substitution was suggested to be responsible for the expansion of the hydrolyzing activity against several broad-spectrum cephalosporins, and this finding was consistent with the kinetic parameters determined with purified enzymes. These findings suggest that the blaCMY-19 genes found in the four K. pneumoniae isolates might have originated from blaCMY-9 gene following a point mutation and dispersed among genetically different K. pneumoniae isolates via a large transferable plasmid.

* Corresponding author. Mailing address: Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 500. Fax: 81-42-561-7173. E-mail: yarakawa{at}nih.go.jp.

Antimicrobial Agents and Chemotherapy, February 2006, p. 534-541, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.534-541.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kotsakis, S. D., Papagiannitsis, C. C., Tzelepi, E., Tzouvelekis, L. S., Miriagou, V. (2009). Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase. Antimicrob. Agents Chemother. 53: 3520-3523 [Abstract] [Full Text]  
  • Doi, Y., Paterson, D. L., Adams-Haduch, J. M., Sidjabat, H. E., O'Keefe, A., Endimiani, A., Bonomo, R. A. (2009). Reduced Susceptibility to Cefepime among Escherichia coli Clinical Isolates Producing Novel Variants of CMY-2 {beta}-Lactamase. Antimicrob. Agents Chemother. 53: 3159-3161 [Abstract] [Full Text]  
  • Tenover, F. C., Emery, S. L., Spiegel, C. A., Bradford, P. A., Eells, S., Endimiani, A., Bonomo, R. A., McGowan, J. E. Jr. (2009). Identification of Plasmid-Mediated AmpC {beta}-Lactamases in Escherichia coli, Klebsiella spp., and Proteus Species Can Potentially Improve Reporting of Cephalosporin Susceptibility Testing Results. J. Clin. Microbiol. 47: 294-299 [Abstract] [Full Text]  
  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Nagano, Y., Nagano, N., Wachino, J.-i., Ishikawa, K., Arakawa, Y. (2009). Novel Chimeric {beta}-Lactamase CTX-M-64, a Hybrid of CTX-M-15-Like and CTX-M-14 {beta}-Lactamases, Found in a Shigella sonnei Strain Resistant to Various Oxyimino-Cephalosporins, Including Ceftazidime. Antimicrob. Agents Chemother. 53: 69-74 [Abstract] [Full Text]  
  • Sohn, S. G., Lee, J. J., Sohn, E. S., Kang, L.-W., Lee, S. H. (2008). Comment on: Extension of the hydrolysis spectrum of AmpC {beta}-lactamase of Escherichia coli due to amino acid insertion in the H-10 helix. J Antimicrob Chemother 61: 965-966 [Full Text]  
  • Vimont, S., Aubert, D., Mazoit, J.-X., Poirel, L., Nordmann, P. (2007). Broad-spectrum {beta}-lactams for treating experimental peritonitis in mice due to Escherichia coli producing plasmid-encoded cephalosporinases. J Antimicrob Chemother 60: 1045-1050 [Abstract] [Full Text]  
  • Hee Lee, S., Lee, J. H., Heo, M. J., Bae, I. K., Jeong, S. H., Cha, S.-S., Wachino, J.-i., Arakawa, Y. (2007). Exact Location of the Region Responsible for the Extended Substrate Spectrum in Class C {beta}-Lactamases. Antimicrob. Agents Chemother. 51: 3778-3779 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»