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Antimicrobial Agents and Chemotherapy, April 2006, p. 1282-1286, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1282-1286.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

In Vitro Analysis of ISEcp1B-Mediated Mobilization of Naturally Occurring ß-Lactamase Gene blaCTX-M of Kluyvera ascorbata

Marie-Frédérique Lartigue, Laurent Poirel, Daniel Aubert, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K-Bicêtre, France

Received 14 November 2005/ Returned for modification 13 December 2005/ Accepted 11 January 2006

ISEcp1B has been reported to be associated with and to mobilize the emerging expanded-spectrum ß-lactamase blaCTX-M genes in Enterobacteriaceae. Thus, the ability of this insertion sequence to mobilize the blaCTX-M-2 gene was tested from its progenitor, Kluyvera ascorbata. Insertions of ISEcp1B upstream of the blaCTX-M-2 gene in K. ascorbata reference strain CIP7953 were first selected with cefotaxime (0.5 and 2 µg/ml). In those cases, ISEcp1B brought promoter sequences enhancing blaCTX-M-2 expression in K. ascorbata. Then, ISEcp1B-mediated mobilization of the blaCTX-M-2 gene from K. ascorbata to Escherichia coli J53 was attempted. The transposition frequency of ISEcp1B-blaCTX-M-2 occurred at (6.4 ± 0.5) x 10–7 in E. coli. Cefotaxime, ceftazidime, and piperacillin enhanced transposition, whereas amoxicillin, cefuroxime, and nalidixic acid did not. Transposition was also enhanced when studied at 40°C.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 K.-Bicêtre, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, April 2006, p. 1282-1286, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1282-1286.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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