This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Powers, J.-P. S.
Right arrow Articles by Hancock, R. E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Powers, J.-P. S.
Right arrow Articles by Hancock, R. E. W.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2006, p. 1522-1524, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1522-1524.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Antimicrobial Peptide Polyphemusin Localizes to the Cytoplasm of Escherichia coli following Treatment

Jon-Paul S. Powers, Morgan M. Martin, Danika L. Goosney, and Robert E. W. Hancock*

Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada

Received 1 November 2005/ Returned for modification 21 December 2005/ Accepted 7 February 2006

The horseshoe crab peptide polyphemusin I possesses high antimicrobial activity, but its mechanism of action is as yet not well defined. Using a biotin-labeled polyphemusin I analogue and confocal fluorescence microscopy, we showed that the peptide accumulates in the cytoplasm of wild-type Escherichia coli within 30 min after addition without causing substantial membrane damage.

* Corresponding author. Mailing address: Centre for Microbial Diseases and Immunity Research, University of British Columbia, Lower Mall Research Station, 2259 Lower Mall, Vancouver, British Columbia V6T 1Z4, Canada. Phone: (604) 822-2682. Fax: (604) 827-5566. E-mail: bob{at}cmdr.ubc.ca.

Antimicrobial Agents and Chemotherapy, April 2006, p. 1522-1524, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1522-1524.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Benincasa, M., Pacor, S., Gennaro, R., Scocchi, M. (2009). Rapid and Reliable Detection of Antimicrobial Peptide Penetration into Gram-Negative Bacteria Based on Fluorescence Quenching. Antimicrob. Agents Chemother. 53: 3501-3504 [Abstract] [Full Text]  
  • Andra, J., Monreal, D., de Tejada, G. M., Olak, C., Brezesinski, G., Gomez, S. S., Goldmann, T., Bartels, R., Brandenburg, K., Moriyon, I. (2007). Rationale for the Design of Shortened Derivatives of the NK-lysin-derived Antimicrobial Peptide NK-2 with Improved Activity against Gram-negative Pathogens. J. Biol. Chem. 282: 14719-14728 [Abstract] [Full Text]  
  • Munoz, A., Lopez-Garcia, B., Marcos, J. F. (2006). Studies on the Mode of Action of the Antifungal Hexapeptide PAF26. Antimicrob. Agents Chemother. 50: 3847-3855 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»