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Antimicrobial Agents and Chemotherapy, April 2006, p. 1586-1589, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1586-1589.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

New Approach for High-Throughput Screening of Drug Activity on Plasmodium Liver Stages

Audrey Gego,1,2,{dagger} Olivier Silvie,1,2,{dagger} Jean-François Franetich,1,2 Khemaïs Farhati,1,2 Laurent Hannoun,2,3 Adrian J. F. Luty,4 Robert W. Sauerwein,4 Claude Boucheix,5,6,7 Eric Rubinstein,5,6,7 and Dominique Mazier1,2,8*

Inserm, U511, F-75013 Paris, France,1 Université Pierre & Marie Curie, Faculté de Médecine Pitié-Salpêtrière, F-75013 Paris, France,2 AP HP, Centre Hospitalo-Universitaire Pitié-Salpêtrière, Service de Chirurgie Digestive, Hépato-Bilio-Pancréatique et Transplantation Hépatique, F-75013 Paris, France,3 University Medical Center St Radboud, Department of Medical Microbiology, 6500HB Nijmegen, The Netherlands,4 Institut André Lwoff, IFR 89, F-94807 Villejuif, France,5 Inserm, U602, F-94807 Villejuif, France,6 Université Paris 11, F-94807 Villejuif, France,7 AP HP, Centre Hospitalo-Universitaire Pitié-Salpêtrière, F-75013 Paris, France8

Received 10 October 2005/ Returned for modification 23 November 2005/ Accepted 6 January 2006

Plasmodium liver stages represent potential targets for antimalarial prophylactic drugs. Nevertheless, there is a lack of molecules active on these stages. We have now developed a new approach for the high-throughput screening of drug activity on Plasmodium liver stages in vitro, based on an infrared fluorescence scanning system. This method allowed us to count automatically and rapidly Plasmodium-infected hepatocytes, using different hepatic cells and different Plasmodium species, including Plasmodium falciparum. This new technique is well adapted for high-throughput drug screening and should facilitate the identification of new antimalarial compounds active on Plasmodium liver stages.

* Corresponding author. Mailing address: Inserm U511, Hôpital Pitié-Salpêtrière, 91 Blvd. de l'Hôpital, 75013 Paris, France. Phone: 33-1-4582-2859. Fax: 33-1-4582-2859. E-mail: mazier{at}ext.jussieu.fr.

{dagger} A.G. and O.S. contributed equally to this study.

Antimicrobial Agents and Chemotherapy, April 2006, p. 1586-1589, Vol. 50, No. 4
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.4.1586-1589.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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