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Antimicrobial Agents and Chemotherapy, May 2006, p. 1664-1671, Vol. 50, No. 5
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.5.1664-1671.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Calcium Signals Are Affected by Ciprofloxacin as a Consequence of Reduction of Mitochondrial DNA Content in Jurkat Cells

Rafal Koziel, Krzysztof Zablocki,* and Jerzy Duszynski

Nencki Institute of Experimental Biology, Pasteura 3, 02-093 Warsaw, Poland

Received 2 December 2005/ Returned for modification 10 January 2006/ Accepted 10 February 2006

The effects of ciprofloxacin on mitochondrial DNA (mtDNA) content, oxygen consumption, mitochondrial membrane potential, cellular ATP formation, and capacitative Ca2+ entry into Jurkat cells were investigated. In cells incubated for several days with 25 µg/ml ciprofloxacin, a 60% reduction of mtDNA content, inhibition of the respiratory chain, and a significant decrease in mitochondrial membrane potential were observed. These changes led to a decrease in the calcium buffering capacity of mitochondria which, in turn, resulted in a gradual inhibition of the capacitative Ca2+ entry. On days 4, 7, and 11 of incubation with ciprofloxacin, the initial rates of Ca2+ entry were reduced by 33%, 50%, and 50%, respectively. Ciprofloxacin caused a transient decrease in the cellular capability for ATP formation. In cells incubated for 15 min with glucose, pyruvate, and glutamine as exogenous fuel, ciprofloxacin reduced ATP content by 16% and 35% on days 4 and 7, respectively, of incubation with the drug. However, on day 11 of incubation with ciprofloxacin, a recovery of cellular ATP formation was observed. In conclusion, long-term exposure of Jurkat cells to ciprofloxacin at a concentration of 25 µg/ml seriously affects cellular energy metabolism and calcium homeostasis.

* Corresponding author. Mailing address: Nencki Institute of Experimental Biology, Pasteura 3, 02-093 Warsaw, Poland. Phone: 4822 5892497. Fax: 4822 8225342. E-mail: k.zablocki{at}nencki.gov.pl.

Antimicrobial Agents and Chemotherapy, May 2006, p. 1664-1671, Vol. 50, No. 5
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.5.1664-1671.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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