This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noto, M. J.
Right arrow Articles by Archer, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noto, M. J.
Right arrow Articles by Archer, G. L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 2006, p. 2782-2788, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00032-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Subset of Staphylococcus aureus Strains Harboring Staphylococcal Cassette Chromosome mec (SCCmec) Type IV Is Deficient in CcrAB-Mediated SCCmec Excision{dagger}

Michael J. Noto1 and Gordon L. Archer2*

Departments of Microbiology and Immunology,1 Internal Medicine, Virginia Commonwealth University School of Medicine, McGuire Hall Room 103, 1112 East Clay Street, Richmond, Virginia2

Received 9 January 2006/ Returned for modification 31 March 2006/ Accepted 17 May 2006

The gene encoding resistance to ß-lactam antibiotics in the staphylococci is found on the chromosome in a genomic island designated staphylococcal cassette chromosome mec, or SCCmec. In addition to the resistance gene mecA, SCCmec also contains site-specific recombinase genes that are capable of catalyzing the chromosomal excision and reintegration of SCCmec. SCCmec is found in five major isotypes partially defined by the recombinase genes present, either ccrAB or ccrC. Of these, SCCmec type IV is presumed to be mobile in the environment, and this mobility may be partially responsible for the rise in community-associated methicillin-resistant staphylococcal infections. In this study, we investigate the presumptive first step in type IV SCCmec mobility: chromosomal excision of the element. CcrAB from a panel of six Staphylococcus aureus and four Staphylococcus epidermidis strains were able to catalyze chromosomal excision of SCCmec types I and II, indicating that these proteins maintain recombinase activity despite varying by up to 3.7% at the amino acid level. Excision of type IV SCCmec was not universally seen, as a subset of S. aureus strains with type IV SCCmec did not excise their element. These strains are all highly related and represent a lineage of successful community-associated pathogens. In addition, the inability to excise SCCmec in these strains is associated with the insertion of a presumptive mobile element containing the gene for staphylococcal enterotoxin H (seh) immediately downstream of SCCmec on the chromosome. Acquisition of this mobile element, containing a known virulence gene, appears to have stabilized the chromosomal integration of the methicillin resistance gene in these strains.

* Corresponding author. Mailing address: Virginia Commonwealth University, P.O. Box 980565, 1101 East Marshall Street, Richmond, VA 23298-0565. Phone: (804) 828-0678. Fax: (804) 828-5022. E-mail: garcher{at}vcu.edu.

{dagger} Supplemental material for this article may be found at http://aac.asm.org/.

Antimicrobial Agents and Chemotherapy, August 2006, p. 2782-2788, Vol. 50, No. 8
0066-4804/06/$08.00+0     doi:10.1128/AAC.00032-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Higgins, P. G., Rosato, A. E., Seifert, H., Archer, G. L., Wisplinghoff, H. (2009). Differential Expression of ccrA in Methicillin-Resistant Staphylococcus aureus Strains Carrying Staphylococcal Cassette Chromosome mec Type II and IVa Elements. Antimicrob. Agents Chemother. 53: 4556-4558 [Abstract] [Full Text]  
  • Noto, M. J., Fox, P. M., Archer, G. L. (2008). Spontaneous Deletion of the Methicillin Resistance Determinant, mecA, Partially Compensates for the Fitness Cost Associated with High-Level Vancomycin Resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 52: 1221-1229 [Abstract] [Full Text]  
  • Noto, M. J., Kreiswirth, B. N., Monk, A. B., Archer, G. L. (2008). Gene Acquisition at the Insertion Site for SCCmec, the Genomic Island Conferring Methicillin Resistance in Staphylococcus aureus. J. Bacteriol. 190: 1276-1283 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»