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Antimicrobial Agents and Chemotherapy, September 2006, p. 3142-3145, Vol. 50, No. 9
0066-4804/06/$08.00+0     doi:10.1128/AAC.00342-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Generation of a Reporter Cell Line for Detection of Infectious Varicella-Zoster Virus and Its Application to Antiviral Studies

Guan-Qing Wang,1,{dagger} Tatsuo Suzutani,2 Yumiko Yamamoto,1 Yoshiko Fukui,1 Naoki Nozawa,1 D. Scott Schmid,3 Ichiro Kurane,1 and Naoki Inoue1*

Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan,1 Department of Microbiology, Fukushima Medical University, Fukushima, Japan,2 National Varicella-Zoster Virus Laboratory, Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 22 March 2006/ Returned for modification 27 April 2006/ Accepted 5 July 2006

To simplify the titration of infectious varicella-zoster virus (VZV), we generated a reporter cell line that produced luciferase in a dose-dependent manner upon infection with cell-free VZV. A few VZV-infected cells were detectable by coculturing with the cell line. We demonstrated the usefulness of the cell line for antiviral studies.

* Corresponding author. Mailing address: Laboratory of Herpesviruses, Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640. Phone: 81-3-5285-1111, ext. 2528. Fax: 81-3-5285-1180. E-mail: ninoue{at}nih.go.jp.

{dagger} Present address: Department of Dermatology, Zhongshan Hospital of Xiamen University, and Department of Dermatology, Medical College of Xiamen University, Xiamen, China.

Antimicrobial Agents and Chemotherapy, September 2006, p. 3142-3145, Vol. 50, No. 9
0066-4804/06/$08.00+0     doi:10.1128/AAC.00342-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Che, X., Berarducci, B., Sommer, M., Ruyechan, W. T., Arvin, A. M. (2007). The Ubiquitous Cellular Transcriptional Factor USF Targets the Varicella-Zoster Virus Open Reading Frame 10 Promoter and Determines Virulence in Human Skin Xenografts in SCIDhu Mice In Vivo. J. Virol. 81: 3229-3239 [Abstract] [Full Text]  


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