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Antimicrobial Agents and Chemotherapy, September 2006, p. 3160-3161, Vol. 50, No. 9
0066-4804/06/$08.00+0     doi:10.1128/AAC.00563-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Escape of Candida from Caspofungin Inhibition at Concentrations above the MIC (Paradoxical Effect) Accomplished by Increased Cell Wall Chitin; Evidence for ß-1,6-Glucan Synthesis Inhibition by Caspofungin

David A. Stevens,1* Masayuki Ichinomiya,2,{dagger} Yukako Koshi,2 and Hiroyuki Horiuchi2

Department of Medicine, Santa Clara Valley Medical Center, San Jose, California 95128-2699, and Stanford University, Stanford, California 94305,1 Department of Biotechnology, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan2

Received 7 May 2006/ Returned for modification 13 June 2006/ Accepted 5 July 2006

Concentrations above the MIC of caspofungin allow growth of some Candida isolates. A strain demonstrating paradoxical growth was grown in the presence and absence of caspofungin, and the cell wall content was analyzed. ß-1,3-Glucan declined 81% in the presence of caspofungin, as expected. ß-1,6-Glucan declined 73%. Chitin increased 898%, demonstrating a mechanism for paradoxical growth—a rapid shift in the key polymer.

* Corresponding author. Mailing address: Santa Clara Valley Medical Center, 751 South Bascom Ave., San Jose, CA 95128-2699. Phone: (408) 885-4313. Fax: (408) 885-4306. E-mail: stevens{at}stanford.edu.

{dagger} Present address: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.

Antimicrobial Agents and Chemotherapy, September 2006, p. 3160-3161, Vol. 50, No. 9
0066-4804/06/$08.00+0     doi:10.1128/AAC.00563-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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