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Antimicrobial Agents and Chemotherapy, November 2007, p. 4009-4014, Vol. 51, No. 11
0066-4804/07/$08.00+0     doi:10.1128/AAC.00537-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular and Biochemical Characterization of the Chromosome-Encoded Class A ß-Lactamase BCL-1 from Bacillus clausii{triangledown}

Delphine Girlich,1 Roland Leclercq,2 Thierry Naas,1 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre,1 Service de Microbiologie, Hopital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France2

Received 23 April 2007/ Returned for modification 28 June 2007/ Accepted 30 August 2007

A chromosomal ß-lactamase gene from Bacillus clausii NR, which is used as a probiotic, was cloned and expressed in Escherichia coli. It encodes a clavulanic acid-susceptible Ambler class A ß-lactamase, BCL-1, with a pI of 5.5 and a molecular mass of ca. 32 kDa. It shares 91% and 62% amino acid identity with the chromosomally encoded PenP penicillinases from B. clausii KSM-K16 and Bacillus licheniformis, respectively. The hydrolytic profile of this ß-lactamase includes penicillins, narrow-spectrum cephalosporins, and cefpirome. This chromosome-encoded enzyme was inducible in B. clausii, and its gene is likely related to upstream-located regulatory genes that share significant identity with those reported to be upstream of the penicillinase gene of B. licheniformis. The blaBCL-1 gene was located next to the known chromosomal aadD2 gene and the erm34 gene, which encode resistance to aminoglycosides and macrolides, respectively. Similar genes were found in a collection of B. clausii reference strains.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 K. Bicêtre Cedex, France. Phone: 33 1 45 21 36 32. Fax: 33 1 45 21 63 40. E-mail: nordmann.patrice{at}bct.aphp.fr

{triangledown} Published ahead of print on 10 September 2007.

Antimicrobial Agents and Chemotherapy, November 2007, p. 4009-4014, Vol. 51, No. 11
0066-4804/07/$08.00+0     doi:10.1128/AAC.00537-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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