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Antimicrobial Agents and Chemotherapy, December 2007, p. 4508-4511, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.00317-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Extensive Genetic Diversity in the Plasmodium falciparum Na+/H+ Exchanger 1 Transporter Protein Implicated in Quinine Resistance{triangledown}

Sumiti Vinayak,1 Mohammad Tauqeer Alam,1 Mala Upadhyay,1 Manoj K. Das,2 Vas Dev,3 Neeru Singh,4 Aditya P. Dash,5 and Yagya D. Sharma1*

Department of Biotechnology, All India Institute of Medical Sciences, New Delhi,1 National Institute of Malaria Research, Field Station Car Nicobar, Andaman and Nicobar Islands,2 Field Station Sonapur, National Institute of Malaria Research, Assam,3 Field Station Jabalpur, National Institute of Malaria Research, Madhya Pradesh,4 National Institute of Malaria Research, Delhi, India5

Received 7 March 2007/ Returned for modification 22 July 2007/ Accepted 26 September 2007

The Plasmodium falciparum Na+/H+ exchanger (Pfnhe-1) locus at chromosome 13 and another locus at chromosome 9 have recently been proposed to influence quinine resistance. Here, we sequenced the ms4760 locus of the Pfnhe-1 gene from 244 P. falciparum isolates collected from five different regions of India. A total of 16 different ms4760 alleles (with one to five DNNND repeats) were observed among these isolates. Interestingly, areas with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance showed more Pfnhe-1 DNNND repeats compared to low drug resistance areas. The extent of genetic diversity at the ms4760 locus also varied from one region to another, with expected heterozygosity values ranging from 0.47 to 0.88.

* Corresponding author. Mailing address: Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. Phone: 91-11-26588145. Fax: 91-11-26589286. E-mail: ydsharma_aiims{at}yahoo.com

{triangledown} Published ahead of print on 8 October 2007.

Antimicrobial Agents and Chemotherapy, December 2007, p. 4508-4511, Vol. 51, No. 12
0066-4804/07/$08.00+0     doi:10.1128/AAC.00317-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Henry, M., Briolant, S., Zettor, A., Pelleau, S., Baragatti, M., Baret, E., Mosnier, J., Amalvict, R., Fusai, T., Rogier, C., Pradines, B. (2009). Plasmodium falciparum Na+/H+ Exchanger 1 Transporter Is Involved in Reduced Susceptibility to Quinine. Antimicrob. Agents Chemother. 53: 1926-1930 [Abstract] [Full Text]  


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