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Antimicrobial Agents and Chemotherapy, February 2007, p. 673-678, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00802-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Novel Macrolide Resistance Module Carried by the IncP-1ß Resistance Plasmid pRSB111, Isolated from a Wastewater Treatment Plant{triangledown}

Rafael Szczepanowski, Irene Krahn, Nadine Bohn, Alfred Pühler,* and Andreas Schlüter

Fakultät für Biologie, Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany

Received 4 July 2006/ Returned for modification 19 August 2006/ Accepted 30 October 2006

The macrolide resistance plasmid pRSB111 was isolated from bacteria residing in the final effluents of a wastewater treatment plant. The 47-kb plasmid confers resistance to azithromycin, clarithromycin, erythromycin, roxithromycin, and tylosin when it is carried by Pseudomonas sp. strain B13 and is very similar to prototype IncP-1ß plasmid pB3, which was previously isolated from an activated-sludge bacterial community of a wastewater treatment plant. The two plasmids differ in their accessory regions, located downstream of the conjugative transfer module gene traC. Nucleotide sequence analysis of the pRSB111 accessory region revealed that it contains a new macrolide resistance module composed of the genes mphR(E), mph(E), and mrx(E), which putatively encode a transcriptional regulator, a macrolide phosphotransferase, and a transmembrane transport protein, respectively. Analysis of the contributions of the individual genes of the macrolide resistance module revealed that mph(E) and mrx(E) are required for high-level macrolide resistance. The resistance genes are flanked by two insertion sequences, namely, ISPa15 and ISRSB111. Two truncated transposable elements, IS6100 and remnants of a Tn3-like transposon, were identified in the vicinity of ISRSB111. The accessory element of pRSB111 apparently replaced the Tn402-like element present on the sister plasmid, pB3, as suggested by the conservation of Tn402-specific terminal inverted repeats on pRSB111.

* Corresponding author. Mailing address: Fakultät für Biologie, Lehrstuhl für Genetik, Universität Bielefeld, Postfach 100131, D-33501 Bielefeld, Germany. Phone: 49 (0)521 106-2036. Fax: 49 (0)521 106-5626. E-mail: Puehler{at}Genetik.Uni-Bielefeld.DE.

{triangledown} Published ahead of print on 13 November 2006.

Antimicrobial Agents and Chemotherapy, February 2007, p. 673-678, Vol. 51, No. 2
0066-4804/07/$08.00+0     doi:10.1128/AAC.00802-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Szczepanowski, R., Linke, B., Krahn, I., Gartemann, K.-H., Gutzkow, T., Eichler, W., Puhler, A., Schluter, A. (2009). Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics. Microbiology 155: 2306-2319 [Abstract] [Full Text]  
  • Schluter, A., Krahn, I., Kollin, F., Bonemann, G., Stiens, M., Szczepanowski, R., Schneiker, S., Puhler, A. (2007). IncP-1{beta} Plasmid pGNB1 Isolated from a Bacterial Community from a Wastewater Treatment Plant Mediates Decolorization of Triphenylmethane Dyes. Appl. Environ. Microbiol. 73: 6345-6350 [Abstract] [Full Text]  


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