This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doi, Y.
Right arrow Articles by Paterson, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doi, Y.
Right arrow Articles by Paterson, D. L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 2007, p. 852-856, Vol. 51, No. 3
0066-4804/07/$08.00+0     doi:10.1128/AAC.01345-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-ß-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil{triangledown}

Yohei Doi,1* Doroti de Oliveira Garcia,2 Jennifer Adams,1 and David L. Paterson1

Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213,1 Instituto Adolfo Lutz, São Paulo, Brazil2

Received 26 October 2006/ Returned for modification 29 November 2006/ Accepted 30 November 2006

Serious infections with Pseudomonas aeruginosa are frequently treated with the combination of a ß-lactam antimicrobial and an aminoglycoside. P. aeruginosa strain PA0905 was isolated in 2005 from an inpatient in Brazil. It showed a panresistant phenotype that included resistance to ß-lactams, aminoglycosides, and fluoroquinolones. The ß-lactam resistance was conferred by the production of the metallo-ß-lactamase SPM-1. No inhibitory zone was observed when a disk diffusion test was performed with the semisynthetic aminoglycoside arbekacin, raising suspicion of 16S rRNA methylase production. A cloning experiment subsequently revealed the presence of a novel 16S rRNA methylase, RmtD, which accounted for the high-level resistance to all 4,6-disubstituted deoxystreptamine aminoglycosides, such as amikacin, tobramycin, and gentamicin. RmtD shared a moderate degree of identity with RmtA, another 16S rRNA methylase that was initially reported to occur in P. aeruginosa in Japan in 2003. This is the first identification of aminoglycoside resistance mediated by a 16S rRNA methylase in South America. This is also the first report to document coproduction of a metallo-ß-lactamase and a 16S rRNA methylase, a combination that would severely compromise therapeutic options for the infected patients.

* Corresponding author. Mailing address: Division of Infectious Diseases, University of Pittsburgh Medical Center, Falk Medical Building Suite 3A, 3601 Fifth Avenue, Pittsburgh PA 15213. Phone: (412) 648-6401. Fax: (412) 648-6399. E-mail: doiy{at}dom.pitt.edu.

{triangledown} Published ahead of print on 11 December 2006.

Antimicrobial Agents and Chemotherapy, March 2007, p. 852-856, Vol. 51, No. 3
0066-4804/07/$08.00+0     doi:10.1128/AAC.01345-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lister, P. D., Wolter, D. J., Hanson, N. D. (2009). Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms. Clin. Microbiol. Rev. 22: 582-610 [Abstract] [Full Text]  
  • Savic, M., Lovric, J., Tomic, T. I., Vasiljevic, B., Conn, G. L. (2009). Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics. Nucleic Acids Res 37: 5420-5431 [Abstract] [Full Text]  
  • Cubrilo, S., Babic, F., Douthwaite, S., Maravic Vlahovicek, G. (2009). The aminoglycoside resistance methyltransferase Sgm impedes RsmF methylation at an adjacent rRNA nucleotide in the ribosomal A site. RNA 15: 1492-1497 [Abstract] [Full Text]  
  • Wu, Q., Zhang, Y., Han, L., Sun, J., Ni, Y. (2009). Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China. Antimicrob. Agents Chemother. 53: 271-272 [Abstract] [Full Text]  
  • Toleman, M. A., Walsh, T. R. (2008). Evolution of the ISCR3 Group of ISCR Elements. Antimicrob. Agents Chemother. 52: 3789-3791 [Abstract] [Full Text]  
  • Doi, Y., Wachino, J.-i., Arakawa, Y. (2008). Nomenclature of Plasmid-Mediated 16S rRNA Methylases Responsible for Panaminoglycoside Resistance. Antimicrob. Agents Chemother. 52: 2287-2288 [Full Text]  
  • Doi, Y., Adams-Haduch, J. M., Paterson, D. L. (2008). Genetic Environment of 16S rRNA Methylase Gene rmtD. Antimicrob. Agents Chemother. 52: 2270-2272 [Abstract] [Full Text]  
  • Fritsche, T. R., Castanheira, M., Miller, G. H., Jones, R. N., Armstrong, E. S. (2008). Detection of Methyltransferases Conferring High-Level Resistance to Aminoglycosides in Enterobacteriaceae from Europe, North America, and Latin America. Antimicrob. Agents Chemother. 52: 1843-1845 [Abstract] [Full Text]  
  • Yamane, K., Rossi, F., Barberino, M. G. M. A., Adams-Haduch, J. M., Doi, Y., Paterson, D. L. (2008). 16S ribosomal RNA methylase RmtD produced by Klebsiella pneumoniae in Brazil. J Antimicrob Chemother 61: 746-747 [Full Text]  
  • Zong, Z., Partridge, S. R., Iredell, J. R. (2008). RmtC 16S rRNA Methyltransferase in Australia. Antimicrob. Agents Chemother. 52: 794-795 [Full Text]  
  • Wachino, J.-i., Shibayama, K., Kurokawa, H., Kimura, K., Yamane, K., Suzuki, S., Shibata, N., Ike, Y., Arakawa, Y. (2007). Novel Plasmid-Mediated 16S rRNA m1A1408 Methyltransferase, NpmA, Found in a Clinically Isolated Escherichia coli Strain Resistant to Structurally Diverse Aminoglycosides. Antimicrob. Agents Chemother. 51: 4401-4409 [Abstract] [Full Text]  
  • Doi, Y., Ghilardi, A. C. R., Adams, J., de Oliveira Garcia, D., Paterson, D. L. (2007). High Prevalence of Metallo-{beta}-Lactamase and 16S rRNA Methylase Coproduction among Imipenem-Resistant Pseudomonas aeruginosa Isolates in Brazil. Antimicrob. Agents Chemother. 51: 3388-3390 [Abstract] [Full Text]  
  • Perichon, B., Courvalin, P., Galimand, M. (2007). Transferable Resistance to Aminoglycosides by Methylation of G1405 in 16S rRNA and to Hydrophilic Fluoroquinolones by QepA-Mediated Efflux in Escherichia coli. Antimicrob. Agents Chemother. 51: 2464-2469 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»