This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, J.-J.
Right arrow Articles by Yan, J.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, J.-J.
Right arrow Articles by Yan, J.-J.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2007, p. 1223-1227, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01195-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Prevalence of Plasmid-Mediated Quinolone Resistance Determinants QnrA, QnrB, and QnrS among Clinical Isolates of Enterobacter cloacae in a Taiwanese Hospital{triangledown}

Jiunn-Jong Wu,1 Wen-Chien Ko,2 Shu-Huei Tsai,3 and Jing-Jou Yan3,4*

Departments of Medical Laboratory Science and Biotechnology,1 Internal Medicine,2 Pathology, College of Medicine, National Cheng Kung University,3 Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan, Republic of China4

Received 23 September 2006/ Returned for modification 11 December 2006/ Accepted 15 January 2007

The prevalence of three plasmid-mediated quinolone resistance determinants, QnrA, QnrB, and QnrS, among 526 nonreplicate clinical isolates of Enterobacter cloacae collected at a Taiwanese university hospital in 2004 was determined by PCR and colony hybridization, and the association of Qnr with the IMP-8 metallo-ß-lactamase was investigated. Eighty-six (16.3%) of all isolates were qnr positive, and the qnrA1-like, qnrB2-like, and qnrS1-like genes were detected alone or in combination in 3 (0.6%), 53 (10.1%), and 34 (6.5%) isolates, respectively. Among 149 putative extended-spectrum-ß-lactamase-producing isolates, 59 (39.6%) isolates, all of which were SHV-12 producers, harbored qnrA (0.7%; 1 isolate), qnrB (28.9%; 43 isolates), or qnrS (12.1%; 18 isolates). Forty-four (78.6%) of 56 IMP-8 producers carried qnrB (58.9%; 33 isolates), qnrS (25.0%; 14 isolates), or both. PCR and sequence analysis revealed that qnrA1 was located in a complex sul1-type integron that contains dhr15, aadA2, qacE{Delta}1, sul1, orf513, qnrA1, ampR, and qacE{Delta}1. Conjugation experiments revealed the coexistence of qnrB and blaIMP-8 on the transferred plasmids and the absence of ß-lactamase content on the transferred qnrS-positive plasmids. The transferred blaIMP-8-positive plasmids with and without qnrB had very similar restriction patterns, suggesting the horizontal mobility of qnrB. Pulsed-field gel electrophoresis showed six major patterns among the 44 qnr-positive IMP-8-producing isolates. Thus, the extremely high prevalence of qnr among the metallo-ß-lactamase-producing E. cloacae isolates in the hospital may be due mainly to the intrahospital spread of a few clones and the dissemination of plasmids containing both qnrB and blaIMP-8.

* Corresponding author. Mailing address: Department of Pathology, National Cheng Kung University Hospital, 138 Sheng-Li Road, Tainan 70428, Taiwan, Republic of China. Phone: 886-6-2353535, ext. 2634. Fax: 886-6-2766195. E-mail: jingjou{at}mail.ncku.edu.tw

{triangledown} Published ahead of print on 22 January 2007.

Antimicrobial Agents and Chemotherapy, April 2007, p. 1223-1227, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01195-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Strahilevitz, J., Jacoby, G. A., Hooper, D. C., Robicsek, A. (2009). Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat. Clin. Microbiol. Rev. 22: 664-689 [Abstract] [Full Text]  
  • Chen, Y.-T., Liao, T.-L., Liu, Y.-M., Lauderdale, T.-L., Yan, J.-J., Tsai, S.-F. (2009). Mobilization of qnrB2 and ISCR1 in Plasmids. Antimicrob. Agents Chemother. 53: 1235-1237 [Abstract] [Full Text]  
  • Ma, J., Zeng, Z., Chen, Z., Xu, X., Wang, X., Deng, Y., Lu, D., Huang, L., Zhang, Y., Liu, J., Wang, M. (2009). High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants qnr, aac(6')-Ib-cr, and qepA among Ceftiofur-Resistant Enterobacteriaceae Isolates from Companion and Food-Producing Animals. Antimicrob. Agents Chemother. 53: 519-524 [Abstract] [Full Text]  
  • Kim, H. B., Park, C. H., Kim, C. J., Kim, E.-C., Jacoby, G. A., Hooper, D. C. (2009). Prevalence of Plasmid-Mediated Quinolone Resistance Determinants over a 9-Year Period. Antimicrob. Agents Chemother. 53: 639-645 [Abstract] [Full Text]  
  • Shen, P., Jiang, Y., Zhou, Z., Zhang, J., Yu, Y., Li, L. (2008). Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae. J Antimicrob Chemother 62: 1252-1256 [Abstract] [Full Text]  
  • Wu, J.-J., Ko, W.-C., Chiou, C.-S., Chen, H.-M., Wang, L.-R., Yan, J.-J. (2008). Emergence of Qnr determinants in human Salmonella isolates in Taiwan. J Antimicrob Chemother 62: 1269-1272 [Abstract] [Full Text]  
  • Yang, H., Chen, H., Yang, Q., Chen, M., Wang, H. (2008). High Prevalence of Plasmid-Mediated Quinolone Resistance Genes qnr and aac(6')-Ib-cr in Clinical Isolates of Enterobacteriaceae from Nine Teaching Hospitals in China. Antimicrob. Agents Chemother. 52: 4268-4273 [Abstract] [Full Text]  
  • Tamang, M. D., Seol, S. Y., Oh, J.-Y., Kang, H. Y., Lee, J. C., Lee, Y. C., Cho, D. T., Kim, J. (2008). Plasmid-Mediated Quinolone Resistance Determinants qnrA, qnrB, and qnrS among Clinical Isolates of Enterobacteriaceae in a Korean Hospital. Antimicrob. Agents Chemother. 52: 4159-4162 [Abstract] [Full Text]  
  • Rice, L. B., Carias, L. L., Hutton, R. A., Rudin, S. D., Endimiani, A., Bonomo, R. A. (2008). The KQ Element, a Complex Genetic Region Conferring Transferable Resistance to Carbapenems, Aminoglycosides, and Fluoroquinolones in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 52: 3427-3429 [Abstract] [Full Text]  
  • Liu, J.-H., Deng, Y.-T., Zeng, Z.-L., Gao, J.-H., Chen, L., Arakawa, Y., Chen, Z.-L. (2008). Coprevalence of Plasmid-Mediated Quinolone Resistance Determinants QepA, Qnr, and AAC(6')-Ib-cr among 16S rRNA Methylase RmtB-Producing Escherichia coli Isolates from Pigs. Antimicrob. Agents Chemother. 52: 2992-2993 [Full Text]  
  • Sanchez-Cespedes, J., Blasco, M. D., Marti, S., Alba, V., Alcalde, E., Esteve, C., Vila, J. (2008). Plasmid-Mediated QnrS2 Determinant from a Clinical Aeromonas veronii Isolate. Antimicrob. Agents Chemother. 52: 2990-2991 [Full Text]  
  • Chmelnitsky, I., Navon-Venezia, S., Strahilevitz, J., Carmeli, Y. (2008). Plasmid-Mediated qnrB2 and Carbapenemase Gene blaKPC-2 Carried on the Same Plasmid in Carbapenem-Resistant Ciprofloxacin-Susceptible Enterobacter cloacae Isolates. Antimicrob. Agents Chemother. 52: 2962-2965 [Abstract] [Full Text]  
  • Iabadene, H., Messai, Y., Ammari, H., Ramdani-Bouguessa, N., Lounes, S., Bakour, R., Arlet, G. (2008). Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in Algeria. J Antimicrob Chemother 62: 133-136 [Abstract] [Full Text]  
  • Wu, J.-J., Ko, W.-C., Wu, H.-M., Yan, J.-J. (2008). Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005. J Antimicrob Chemother 61: 1234-1239 [Abstract] [Full Text]  
  • Jiang, Y., Zhou, Z., Qian, Y., Wei, Z., Yu, Y., Hu, S., Li, L. (2008). Plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in extended-spectrum {beta}-lactamase-producing Escherichia coli and Klebsiella pneumoniae in China. J Antimicrob Chemother 61: 1003-1006 [Abstract] [Full Text]  
  • Yamane, K., Wachino, J.-i., Suzuki, S., Arakawa, Y. (2008). Plasmid-Mediated qepA Gene among Escherichia coli Clinical Isolates from Japan. Antimicrob. Agents Chemother. 52: 1564-1566 [Abstract] [Full Text]  
  • Aschbacher, R., Doumith, M., Livermore, D. M., Larcher, C., Woodford, N. (2008). Linkage of acquired quinolone resistance (qnrS1) and metallo-{beta}-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy. J Antimicrob Chemother 61: 515-523 [Abstract] [Full Text]  
  • Mendes, R. E., Bell, J. M., Turnidge, J. D., Yang, Q., Yu, Y., Sun, Z., Jones, R. N. (2008). Carbapenem-Resistant Isolates of Klebsiella pneumoniae in China and Detection of a Conjugative Plasmid (blaKPC-2 plus qnrB4) and a blaIMP-4 Gene. Antimicrob. Agents Chemother. 52: 798-799 [Full Text]  
  • Xu, X., Wu, S., Ye, X., Liu, Y., Shi, W., Zhang, Y., Wang, M. (2007). Prevalence and Expression of the Plasmid-Mediated Quinolone Resistance Determinant qnrA1. Antimicrob. Agents Chemother. 51: 4105-4110 [Abstract] [Full Text]  
  • Ahmed, A. M., Motoi, Y., Sato, M., Maruyama, A., Watanabe, H., Fukumoto, Y., Shimamoto, T. (2007). Zoo Animals as Reservoirs of Gram-Negative Bacteria Harboring Integrons and Antimicrobial Resistance Genes. Appl. Environ. Microbiol. 73: 6686-6690 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»