This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zolezzi, P. C.
Right arrow Articles by Gómez-Lus, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zolezzi, P. C.
Right arrow Articles by Gómez-Lus, R.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2007, p. 1487-1490, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01374-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Macrolide and Tetracycline Resistances in Commensal Gemella sp. Isolates{triangledown}

Paula Cerdá Zolezzi,1 Pilar Goñi Cepero,1 Joaquim Ruiz,2 Leticia Millán Laplana,1 Carmen Rubio Calvo,1,3 and Rafael Gómez-Lus1*

Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009 Zaragoza,1 Centro de Salud Internacional, IDIBAPS, Hospital Clinic, Villarroel 170, 08036 Barcelona,2 Service of Microbiology, Hospital Clínico Lozano Blesa, C/San Juan Bosco 19, 50009 Zaragoza, Spain3

Received 3 November 2006/ Returned for modification 7 December 2006/ Accepted 19 January 2007

The epidemiologic relatedness of 29 erythromycin-resistant Gemella sp. strains from normal flora, characterized previously, were evaluated by pulsed-field gel electrophoresis (PFGE). Three isolates carried the tet(O) gene and the tet(M) gene. The msr(A) gene was found in two Gemella morbillorum strains in combination with the erm(B) or mef(E) gene. The sequences of the mef(A/E), erm(B), and msr(A) genes showed a high similarity to the corresponding sequences of other gram-positive cocci. All the strains harboring the mef(A/E) gene and the msr(D) gene possessed open reading frame 3 (ORF3)/ORF6. The 16 G. morbillorum isolates represented 15 distinct DNA profiles. Four clusters were identified (≥80% genetic relatedness). The 12 Gemella haemolysans strains belonged to different PFGE types. The clonal diversity found suggests that horizontal transfer may be the main route through which erythromycin resistance is acquired.

* Corresponding author. Mailing address: Department of Microbiology, Preventive Medicine and Public Health, Faculty of Medicine, University of Zaragoza, C/Domingo Miral s/n, 50009 Zaragoza, Spain. Phone: 34-976-761692. Fax: 34-976-761693. E-mail: gomezlus{at}unizar.es

{triangledown} Published ahead of print on 5 February 2007.

Antimicrobial Agents and Chemotherapy, April 2007, p. 1487-1490, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01374-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sekirov, I., Tam, N. M., Jogova, M., Robertson, M. L., Li, Y., Lupp, C., Finlay, B. B. (2008). Antibiotic-Induced Perturbations of the Intestinal Microbiota Alter Host Susceptibility to Enteric Infection. Infect. Immun. 76: 4726-4736 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»